Determination of Anthocyanins in Ruscus aculeatus L. Berries

Department of Engineering for Innovation, Università del Salento, Lecce, Apulia, Italy
Journal of Agricultural and Food Chemistry (Impact Factor: 2.91). 02/2005; 53(2):475-9. DOI: 10.1021/jf0487250
Source: PubMed


Red berries of Ruscus aculeatus L., a wild shrub typical of Mediterranean Europe and Africa, were investigated for the first time in order to determine the profile of anthocyanins. The pigments were extracted from the skins of the berries with 0.1% HCl in methanol, purified using a C-18 solid phase cartridge, and identified by means of high-performance liquid chromatography (HPLC)-diode array detection-mass spectrometry analysis. Information from HPLC profiles, saponification, and acid hydrolysis of the anthocyanins showed that the major anthocyanins were pelargonidin 3-O-rutinoside (64%), pelargonidin 3-O-glucoside (16%), and pelargonidin 3-O-trans-p-coumarylglucoside (13%). The attractive color of R. aculeatus berries and the great abundance of the plant in the south of Italy make these berries a new and promising source of natural colorants.

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    • "Because anthocyanins are unstable compounds, the availability of standards is poor. for this reason, it is very difficult and expensive to obtain standards for all anthocyanins present in the sample, and many authors choose one glycoside as a reference for the calibration (Baj et al., 1983; Versari et al., 1997; LoNgo & VasapoLLo, 2005). This is almost correct because the chromatographic responses of these compounds at wavelengths of around 520 nm are rather similar. "
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    • "Acid concentration of the extraction medium was 0.01N. M3: methanol–water medium acidified with hydrochloric acid. 1 mL of concentrated HCl (37%) was added into 1 L of methanol, as previously described (Longo and Vasapollo, 2005). 2.5. "
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