Article

Applications of zeolite inorganic composites in biotechnology: current state and perspectives.

Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda-shi, Chiba-ken 278-8510, Japan.
Applied Microbiology and Biotechnology (Impact Factor: 3.81). 06/2005; 67(3):306-11. DOI: 10.1007/s00253-004-1782-4
Source: PubMed

ABSTRACT The purpose of this short review is to introduce applications of inorganic composites, zeolites, in biotechnology. Although inorganic chemistry is generally considered distant from biotechnology, the two could be harmoniously integrated for biopolymer chromatography. New chromatographic carriers have been developed based on principles differing from those underlying conventional chromatography. Some can be used for the purification of proteins according to novel physicochemical principles, according to their isoelectric point (pI), molecular weight and shape. The amount of protein adsorbed is related to the pore size of the composites, which can recognize biomolecules with reference to these three parameters. Proteins adsorbed at their pI have been found to be desorbed at the pI by polyethylene glycol, but not by high ionic medium (NaCl), SDS, non-ionic detergents, ATP or urea. Therefore, inorganic composites synthesized in consideration of pore size and three-dimensional structure are suitable as new chromatographic carriers. Selective fractionation of biomaterials including proteins and nucleic acids should provide useful information regarding whether conjugated proteins in a precipitated state can be separated on net charge and whether cells can be directly fractionated in future.

0 Bookmarks
 · 
91 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Analytical characteristics of urease- and butyrylcholinesterase (BuChE)- based ion sensitive field-effect transistor (ISFET) biosensors were investigated by the incorporation of zeolite Beta nanoparticles with varying Si/Al ratios. The results obtained by the zeolite-modified ISFET transducers suggested that the Si/Al ratio strongly influenced the biosensor performances due to the electrostatic interactions among enzyme, substrate, and zeolite surface as well as the nature of the enzymatic reaction. Using relatively small nanoparticles (62.7 ± 10, 76.2 ± 10, and 77.1 ± 10 nm) rather than larger particles, that are widely used in the literature, allow us to produce more homogenous products which will give more control over the quantity of materials used on the electrode surface and ability to change solely Si/Al ratio without changing other parameters such as particle size, pore volume, and surface area. This should enable the investigation of the individual effect of changing acidic and electronic nature of this material on the biosensor characteristics. According to our results, high biosensor sensitivity is evident on nanosize and submicron size particles, with the former resulting in higher performance. The sensitivity of biosensors modified by zeolite particles is higher than that to the protein for both types of biosensors. Most significantly, our results show that the performance of constructed ISFET-type biosensors strongly depends on Si/Al ratio of employed zeolite Beta nanoparticles as well as the type of enzymatic reaction employed. All fabricated biosensors demonstrated high signal reproducibility and stability for both BuChE and urease.
    Journal of Nanoparticle Research 15(5). · 2.18 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: New materials are prerequisite for major breakthrough applications influencing our daily life, and therefore are pivotal for the chemical industry. Nanoporous materials constitute an important class of heterogeneous catalysts as they not only offer higher surface areas and enhanced activity, but also provide shape/size selectivity. As a well-established family of nanoporous materials, zeolites are of paramount importance for the chemical industry as heterogeneous catalysts with shape/size-selective character in various reactions. Zeolites can also play an active role in the quest for raw material change as catalysts providing the required selectivity towards base chemicals. In this contribution, an overview of the current and potential applications of zeolites as catalysts in chemical industry is presented.
    Topics in Catalysis 01/2009; 52(6):888-895. · 2.61 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Nanotechnology, the engineering and art of manipulating matter at the nanoscale (1-100 nm), offers the potential of novel nanomaterials for treatment of surface water, groundwater, and wastewater contaminated by toxic metal ions, organic and inorganic solutes, and microorganisms. Due to their unique activity toward recalcitrant contaminants and application flexibility, many nanomaterials are under active research and development. Accordingly, literature about current research on different nanomaterials (nanostructured catalytic membranes, nanosorbents, nanocatalysts, and bioactive nanoparticles) and their application in water treatment, purification and disinfection is reviewed in this article. Moreover, knowledge regarding toxicological effects of engineered nanomaterials on humans and the environment is presented.
    Critical Reviews in Microbiology 02/2008; 34(1):43-69. · 6.09 Impact Factor