Rapid determination of tramadol in human plasma by headspace solid-phase microextraction and capillary gas chromatography-mass spectrometry

Department of Chemistry, Fudan University, Shanghai, Shanghai Shi, China
Journal of Pharmaceutical and Biomedical Analysis (Impact Factor: 2.83). 03/2005; 37(1):143-7. DOI: 10.1016/j.jpba.2004.09.050
Source: PubMed

ABSTRACT A simple, rapid and sensitive method for determination of tramadol in plasma samples was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography with mass spectrometry (GC-MS). The optimum conditions for the SPME procedure were: headspace extraction on a 65-microm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber; 0.5 mL of plasma modified with 0.5 mL of sodium hydroxide (0.1 M); extraction temperature of 100 degrees C, with stirring at 2000 rpm for 30 min. The calibration curve showed linearity in the range of 1-400 ng mL(-1) with regression coefficient corresponding to 0.9986 and coefficient of the variation of the points of the calibration curve lower than 10%. The detection limit for tramadol in plasma was 0.2 ng mL(-1). The proposed method was successfully applied to determination of tramadol in human plasma samples from 10 healthy volunteers after a single oral administration.

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    • "Several analytical methods such as gas chromatography (GC) coupled to nitrogen selective or even mass-spectrometry detection [3], capillary electrophoresis [4] and high performance liquid chromatography (HPLC) with electrochemical [5], mass spectrometry [6] or fluorescence detectors [7] have been introduced for separation and determination of tramadol. Usually, an initial sample preparation step is essential for isolation and preconcentration of tramadol in biological samples prior to its final analysis. "
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    Journal of Chromatography A 12/2011; 1222:5-12. DOI:10.1016/j.chroma.2011.11.055 · 4.26 Impact Factor
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