Article

Effect of systemic vitamin C on free fatty acid-induced lipid peroxidation.

Department of Clinical Pharmacology, Medical University of Vienna, Austria.
Diabetes & Metabolism (impact factor: 2.41). 12/2004; 30(5):433-9.
Source: PubMed

ABSTRACT Plasma malondialdehyde (MDA), a reactive product of lipid peroxidation, may be influenced by anti-oxidant therapy. The aim of the present study was to investigate if elevated MDA as induced by increased free fatty acids (FFA) correlates with endothelial function and is affected by high doses of vitamin C.
The study design was randomised, placebo-controlled, double blind, 2-way cross over. Plasma MDA concentrations and forearm blood flow (FBF) responses to intra-arterial acetylcholine (ACh) and glyceryl trinitrate were assessed during co-administration of vitamin C or placebo in the presence of increased plasma FFA by Intralipid/heparin infusion in 10 healthy male subjects.
The seven-fold rise in plasma FFA was associated with an increase in plasma MDA concentrations (r=0.7, p<0.001) and decreased FBF responses to ACh (r=-0.4, p<0.01). Co-administration of vitamin C restored the impaired reactivity of FBF to ACh but had no effect on elevated MDA concentrations.
Anti-oxidant vitamin C improves lipid-induced impairment of endothelium-dependent vasodilation, but does not alter MDA formation or breakdown.

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    Article: Heme arginate improves reperfusion patterns after ischemia: a randomized, placebo-controlled trial in healthy male subjects.
    Martin Andreas, Albrecht Ingo Schmid, Daniel Doberer, Kiril Schewzow, Stefan Weisshaar, Georg Heinze, Martin Bilban, Ewald Moser, Michael Wolzt
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    ABSTRACT: Heme arginate can induce heme oxygenase-1 to protect tissue against ischemia-reperfusion injury. Blood oxygen level dependent (BOLD) functional magnetic resonance imaging measures changes in tissue oxygenation with a high spatial and temporal resolution. BOLD imaging was applied to test the effect of heme arginate on experimental ischemia reperfusion injury in the calf muscles. A two period, controlled, observer blinded, crossover trial was performed in 12 healthy male subjects. Heme arginate (1 mg/kg body weight) or placebo were infused 24 h prior to a 20 min leg ischemia induced by a thigh cuff. 3 Tesla BOLD-imaging of the calf was performed and signal time courses from soleus, gastrocnemius and tibialis anterior muscle were available from 11 participants for technical reasons. Peak reactive hyperemia signal of the musculature was significantly increased and occurred earlier after heme arginate compared to placebo (106.2±0.6% at 175±16s vs. 104.5±0.6% at 221±19s; p = 0.025 for peak reperfusion and p = 0.012 for time to peak). A single high dose of heme arginate improves reperfusion patterns during ischemia reperfusion injury in humans. BOLD sensitive, functional MRI is applicable for the assessment of experimental ischemia reperfusion injury in skeletal muscle. ClinicalTrials: NCT01461512EudraCT: 2008-006967-35.
    Journal of Cardiovascular Magnetic Resonance 08/2012; 14:55. · 3.72 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

10 healthy male subjects
 
anti-oxidant therapy
 
Anti-oxidant vitamin C
 
endothelial function
 
endothelium-dependent vasodilation
 
forearm blood flow
 
free fatty acids
 
impaired reactivity
 
intra-arterial acetylcholine
 
Intralipid/heparin infusion
 
lipid peroxidation
 
lipid-induced impairment
 
MDA concentrations
 
MDA formation
 
plasma FFA
 
Plasma malondialdehyde
 
Plasma MDA concentrations
 
reactive product
 
seven-fold rise
 
vitamin C