Article

Rapid separation of microorganisms by quartz microchip capillary electrophoresis.

Textile Industrial Institute of Ehime Prefecture, 2-5-48 Higashimura-minami Imabari, Ehime 799-1507, Japan.
Analytical Sciences (impact factor: 1.25). 02/2005; 21(1):57-60.
Source: PubMed

ABSTRACT We developed and optimized a system coupling microchip capillary electrophoresis (MCE) and laser-induced fluorescence (LIF) detection for the analysis of microorganisms. The MCE-LIF system successfully separated pure cultures of lactic acid bacteria and Saccharomyces cerevisiae within 200 s. The results indicate that the MCE system can be conveniently used for the rapid and highly sensitive detection of microorganisms. Thus, MCE can provide a cheap and simple method for the on-line detection of microbial contamination.

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Keywords

laser-induced fluorescence
 
LIF
 
MCE
 
MCE system
 
MCE-LIF system
 
microbial contamination
 
pure cultures
 
sensitive detection
 
simple method
 
system coupling microchip capillary electrophoresis
 

Tomoyoshi Shintani