Article

Digoxigenin labelling and laser capture microdissection of male cells.

Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7a, 80337 Munich, Germany.
Deutsche Zeitschrift für die Gesamte Gerichtliche Medizin (impact factor: 2.59). 11/2005; 119(6):374-7. DOI:10.1007/s00414-005-0523-2 pp.374-7
Source: PubMed

ABSTRACT Laser capture microdissection (LMD) is a relatively new technique for the isolation of single cells. The application in forensic investigations has become more and more widespread, especially to select spermatozoa out of mixtures with vaginal cells. In particular in cases with low numbers of sperm it could be profitable to isolate all male cells (e.g. sperm and male epithelial cells) instead of focussing on the sperm only. Therefore, the specific labelling and detection of the male cells in a male/female cell mixture is necessary. In order to label all cells carrying a Y-chromosome we used a digoxigenin labelled chromosome Y hybridisation probe (Q Biogen). The stained cells were isolated with the SL microCut LMD system from Molecular Machines & Industries AG (MMI). At least ten diploid male cells were required to obtain a partial STR profile, with 20 cells, a full profile could be obtained.

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Keywords

digoxigenin labelled chromosome Y hybridisation probe
 
diploid male cells
 
full profile
 
Laser capture microdissection
 
low numbers
 
male cells
 
male epithelial cells
 
male/female cell mixture
 
mixtures
 
Molecular Machines & Industries AG
 
partial STR profile
 
Q Biogen
 
single cells
 
SL microCut LMD system
 
specific labelling
 
sperm
 
spermatozoa
 
stained cells
 
vaginal cells
 
Y-chromosome