Article

[Mechanisms of purified cobra venom factor in preventing hyperacute rejection following discordant liver xenotransplantation in rats].

Institute of Organ Transplantation, Fudan University, Shanghai 200040, China.
Zhonghua yi xue za zhi 12/2004; 84(23):2007-10. pp.2007-10
Source: PubMed

ABSTRACT To investigate the mechanisms of purified cobra venom factor (CVF) in preventing hyperacute rejection (HAR) and prolonging recipient survival following discordant liver xenotransplantation in rats.
All animals were divided into two groups, group I (n = 20), unmodified recipients as control; group II (n = 20), xenograft recipients were treated with 50 microg/kg CVF i.v. on day-1. The pathologic changes of liver were observed and TNF-alpha of blood serum was detected.
Recipient survival after CVF treatment is significantly prolonged compared with unmodified recipients (1.8 +/- 0.6 vs.7.4 +/- 2.1 h, P < 0.01). Histologically, widespread thrombosis, interstitial haemorrhage, C3 deposits on sinusoids and central veins characterized xenografts of the control group. Xenografts of the CVF group showed endothelium swelling and cellular infiltrate, no deposit of C3 was detected.
Purified CVF can prevent guinea pig-to-rat liver xenografts from HAR and extend recipient survival. Preconditioning with CVF, guinea pig-to-rat combination is a useful life-supporting model to explore further mechanisms of discordant liver xenotransplantation.

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Keywords

50 microg/kg CVF i.v
 
blood serum
 
C3 deposits
 
control group
 
CVF group
 
CVF treatment
 
discordant liver xenotransplantation
 
guinea pig-to-rat combination
 
guinea pig-to-rat liver xenografts
 
interstitial haemorrhage
 
pathologic changes
 
prolonging recipient survival
 
purified cobra venom factor
 
Purified CVF
 
Recipient survival
 
unmodified recipients
 
useful life-supporting model
 
widespread thrombosis
 
xenograft recipients
 
xenografts