Comparative Sublethal Toxicity of Nine Pesticides on Olfactory Learning Performances of the Honeybee Apis mellifera

Association de Coordination Technique Agricole, Maison des Agriculteurs, La Tour de Salvagny, France.
Archives of Environmental Contamination and Toxicology (Impact Factor: 1.9). 03/2005; 48(2):242-50. DOI: 10.1007/s00244-003-0262-7
Source: PubMed

ABSTRACT Using a conditioned proboscis extension response (PER) assay, honeybees (Apis mellifera L.) can be trained to associate an odor stimulus with a sucrose reward. Previous studies have shown that observations of conditioned PER were of interest for assessing the behavioral effects of pesticides on the honeybee. In the present study, the effects of sublethal concentrations of nine pesticides on learning performances of worker bees subjected to the PER assay were estimated and compared. Pesticides were tested at three concentrations. The highest concentration of each pesticide corresponded to the median lethal dose value (48-h oral LD50), received per bee and per day, divided by 20. Reduced learning performances were observed for bees surviving treatment with fipronil, deltamethrin, endosulfan, and prochloraz. A lack of behavioral effects after treatment with lambda-cyalothrin, cypermethrin, tau-fluvalinate, triazamate, and dimethoate was recorded. No-observed-effect concentrations (NOECs) for the conditioned PER were derived for the studied pesticides. Our study shows that the PER assay can be used for estimating sublethal effects of pesticides on bees. Furthermore, comparisons of sensitivity as well as the estimation of NOECs, useful for regulatory purposes, are possible.

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Available from: Axel Decourtye, Sep 26, 2015
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    • ") versus other neonicotinoids, and can act synergistically with N. ceranae to kill honey bees in the laboratory (Vidau et al., 2011; Retschnig et al., 2014a). Taufluvalinate has an acute contact toxicity of 0.2 g μg bee −1 , but was reported to have no lethal effect at daily oral doses of 5 or 10 μg bee −1 (Decourtye et al., 2005). "
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    ABSTRACT: Interactions between pesticides and parasites are believed to be responsible for increased mortality of honey bee (Apis mellifera) colonies in the northern hemisphere. Previous efforts have employed experimental approaches using small groups under laboratory conditions to investigate influence of these stressors on honey bee physiology and behaviour, although both the colony level and field conditions play a key role for eusocial honeybees. Here, we challenged honey bee workers under in vivo colony conditions with sub-lethal doses of the neonicotinoid thiacloprid, the miticide tau-fluvalinate, and the endoparasite Nosema ceranae, to investigate potential effects on longevity and behaviour using observation hives. In contrast to previous laboratory studies our results do not suggest interactions among stressors, but rather lone effects of pesticides and the parasite on mortality and behaviour, respectively. These effects appear to be weak due to different outcomes at the two study sites, thereby suggesting that the role of thiacloprid, tau-fluvalinate, and N. ceranae and interactions among them may have been overemphasized. In the future, investigations into the effects of honey bee stressors should prioritize the use of colonies maintained under a variety of environmental conditions in order to obtain more biologically relevant data. This article is protected by copyright. All rights reserved.
    Environmental Microbiology 03/2015; DOI:10.1111/1462-2920.12825 · 6.20 Impact Factor
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    • "This assertion is underpinned by a number of studies that looked into the toxic impacts of various pesticides on Apis mellifera or bumble bees through dose or exposure–mortality response relationships in controlled ecotoxicological experiments (Decourtye et al. 2004; Choudhary et al. 2009; Brittain et al. 2010; Johnson et al. 2010). Apart from dose mortality responses, a number of biomarkers, including fecundity (Cresswell and Laycock 2012; Henry et al. 2012), sub-lethal markers like proboscis extension response/reflex (Decourtye et al. 2005; Han et al. 2010) or homing behaviour (Henry et al. 2012) and physiological markers (Badiou-Bénéteau et al. 2012), have also been used to study the sensitivity of honey bees to pesticide exposure in controlled experiments. However, it should be noted that: a) little is known about the sensitivities of other native Apis bees apart from A. mellifera with respect to any of the biomarkers that have been used so far; and b) there is a large information gap on the response of Electronic supplementary material The online version of this article (doi:10.1007/s13592-014-0308-z) contains supplementary material, which is available to authorized users. "
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    ABSTRACT: Pesticides have been cited as one of the major drivers of pollinator loss. However, little is known about pesticide impacts on natural populations of native honey bee species. This study looked into the effect of pesticides with respect to oxidative stress in the laboratory and in field populations of two native Indian honey bee species (Apis dorsata and A. cerana) by examining a combination of biomarkers, e.g., superoxide dismutase, catalase and xanthine oxidase. A significant upregulation of all three biomarkers was observed in both treated individuals in laboratory experiments and field populations sampled from a pesticide use gradient. This study reports, for the first time, an increase in expression of xanthine oxidase in an invertebrate system (honey bees) exposed to pesticides. Apis cerana / Apis dorsata / enzyme / super-oxide dismutase / catalase / xanthine oxidase / insecticide
    Apidologie 09/2014; 46(1). DOI:10.1007/s13592-014-0308-z · 1.68 Impact Factor
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    • "Studies assessing the toxicity of fipronil to honeybees reported that it is harmful to these insects (Decourtye et al. 2005; Pochi et al. 2012). It can modulate insects' sensitivity to sucrose (Aliouane et al. 2009), affect learning (Decourtye et al. 2005), orientation and foraging behavior (Decourtye et al. 2009), and cause hyperactivity followed by paralysis and death (Barbara et al. 2005; Gunasekara et al. 2007). The results from studies of the oral toxicity of fipronil against the stingless bee Melipona scutellaris showed that these insects exhibited signs of intoxication, such as initial tremors followed by paralysis, before their death (Lourenço et al. 2012). "
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    ABSTRACT: Fipronil is a phenylpyrazole insecticide that is widely used in Brazilian agriculture for pest control. Although honeybees are not targets of fipronil, studies indicate that this pesticide can be harmful to honeybees. To assess the effects of fipronil in the brain of Africanized Apis mellifera workers, this study focused on the toxico-proteome profiling of the brain of newly emerged and aged honeybee workers that were exposed to a sub-lethal dose (10 pg fipronil per day. i.e. (1)/100 of LD50/bee/day during 5 days) of the insecticide. Proteomic analysis identified 25 proteins that were differentially up-regulated or down-regulated when the fipronil-exposed and non-exposed groups were compared. These proteins are potentially related to pathogen susceptibility, neuronal chemical stress, neuronal protein misfolding, and occurrence of apoptosis, ischemia, visual impairment, damaged synapse formation, brain degeneration, memory and learning impairment. The exposure of honeybees to a very low dose of fipronil, even for a short period of time (5 days), was sufficient to cause a series of important neuroproteomic changes in the brains of honeybees.
    Ecotoxicology 08/2014; 23(9). DOI:10.1007/s10646-014-1305-8 · 2.71 Impact Factor
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