Trans-Resveratrol, a natural antioxidant from grapes, increases sperm output in healthy rats

Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, E-08028 Barcelona, Spain.
Journal of Nutrition (Impact Factor: 3.88). 05/2005; 135(4):757-60.
Source: PubMed


trans-Resveratrol was reported to have health benefits including anticarcinogenic effects and protection against cardiovascular disease. One of the mechanisms by which it exerts its action is through modulating the estrogen response systems. Because estrogen is involved in male reproductive biology, we investigated the effect of trans-resveratrol on testis and spermatogenesis. Adult male rats were divided into 2 groups. The treated group was administered by gavage 20 mg/(kg . d) of trans-resveratrol suspended in 10 g/L of carboxymethylcellulose for 90 d, whereas the control group received only carboxymethylcellulose during the same period. The relative weight of testes did not differ between the groups. However, the diameter of the seminiferous tubules was significantly reduced from 437.5 +/- 0.1 mum in the controls to 310.9 +/- 0.1 mum in the resveratrol-treated rats. This decrease was accompanied by a significant increase in tubular density, from 3.20 +/- 0.18 in controls to 6.58 +/- 0.18 tubules/mm(2) in the treated group. Moreover, sperm counts were significantly greater in the resveratrol-treated rats (24.8 +/- 3.30 x 10(7)) than in the control group (14.1 +/- 0.80 x 10(7)), but sperm quality did not differ. Serum concentrations of gonadotrophins and testosterone were significantly higher in the resveratrol-treated group. We identified a novel activity of trans-resveratrol. The daily oral administration of this phytochemical to adult male rats enhanced sperm production by stimulating the hypothalamic-pituitary-gonadal axis, without inducing adverse effects.

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Available from: Joan E. Rodríguez-Gil, May 16, 2014
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    • "[21] Moreover it has been demonstrated that RES administration enhances spermatogenesis by stimulating the hypothalamic-pituitary-gonadal axis without exhibiting adverse effects. [22] Moreover RES supplementation may trigger penile erection and enhance blood testosterone levels, testicular sperm count and epididymal sperm motility. [19] This preliminary body of evidence emphasizes on a significant potential of RES in providing a possible protection to the male reproductive system. "
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    ABSTRACT: Resveratrol (RES) is a natural polyphenol and phytoestrogen exhibiting cardioprotective, anticancer, antibacterial and vasorelaxing properties. It is also a powerful reactive oxygen species (ROS) scavenger and chelating agent. This study was designed to determine the efficiency of RES to reverse the ROS-mediated impairment of the motility, viability and intracellular antioxidant profile of bovine spermatozoa. Spermatozoa were washed out of fresh bovine semen, suspended in 2.9% sodium citrate and subjected to RES treatment (5, 10, 25 and 50 μmol L−1) in the presence or absence of a pro-oxidant, i.e., ferrous ascorbate (FeAA; 150 μmol L−1 FeSO4 and 750 μmol L−1 ascorbic acid) during a 6-h in vitro culture. Spermatozoa motion parameters were assessed using the SpermVision computer-aided sperm analysis (CASA) system. Cell viability was examined with the metabolic activity (MTT) assay, and the nitroblue-tetrazolium (NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro experiments in order to investigate the intracellular activity of superoxide dismutase (SOD), catalase (CAT), as well as the concentrations of glutathione (GSH) and malondialdehyde (MDA). FeAA treatment led to a reduced sperm motility (P P P P P P P P P −1 RES and P −1 RES; P −1 RES proving to be the most effective RES concentration. Our results suggest that RES possesses significant antioxidant properties that may prevent the deleterious effects caused by ROS to spermatozoa, and preserve the fertilization potential of male reproductive cells.
    Journal of Environmental Science and Health Part A 08/2015; 50(14):1-12. DOI:10.1080/10934529.2015.1071153 · 1.16 Impact Factor
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    • "In the central nervous system (CNS), heme oxygenase is a key defensive mechanism of cells exposed to oxidizing agents. It was found that resveratrol increases expression of inducible heme oxygenase by activation of the nuclear factor Nrf2 [13] [32] [62]. Under homeostasis, Nrf2 accumulates in the cytoplasm and forms a complex with the inhibitory protein Keap1. "
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    ABSTRACT: Resveratrol is a natural organic compound, polyphenol, produced naturally by some plants in response to several harmful factors such as attack by pathogens, UV radiation, or increased oxidative stress. Many experiments suggest that it triggers mechanisms that counteract aging-related effects and plays a role in insulin resistance as well. It also possesses beneficial properties such as anti-cancer, anti-inflammatory, blood-sugar-lowering and cardiovascular effects. It is supposed to exhibit an interesting activity in neuroprotection - mainly through activation of sirtuins and counteraction in forming peptide aggregates. Still research is needed to evaluate exactly how resveratrol protects neurons, and to develop new, potential, therapeutic drugs
    12/2014; 4(2). DOI:10.2478/acb-2014-0006
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    • "Some recent in vivo studies in animal models demonstrated that a long-term application of RSV increases the amount of testosterone and gonadotropins in the blood and enhances sperm production by stimulating the hypothalamic–pituitary–gonadal axis without inducing adverse effects.[1920] "
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    ABSTRACT: This study aimed to observe the possible protective effects of resveratrol (RSV) against damage induced by di-n-butylphthalate (DBP), on the ductus epididymis and deferens in rats. SIX GROUPS OF RATS WERE USED IN THE EXPERIMENT: Group 1: Control group; Group 2: Solvent (carboxymethylcellulose (CMC), 10ml/kg); Group 3: 500 mg/kg/day DBP; Group 4: 500 mg/kg/day DBP+20 mg/kg/day RSV; Group 5: 1000 mg/kg/day DBP; Group 6: 1000mg/kg/day DBP + 20 mg/kg/day RSV. Groups were treated by gavage for 30 days. Immunohistochemical, electronmicroscopic and histomorphometric examinations were carried out in the epididymis and deferens. In the ductus epididymis and deferens mitochondrial crystolysis, exfoliation of the stereocilia and openings in lateral surface increased with DBP dosage, but these structures were recovered with RSV. DBP reduced the epithelial height of epididymis and vas deferens. Lumen dilatation was observed in both tissues. These disorders may lead to dysfunction of epithelial absorption. In the TUNEL examinations in both tissues, there were no apoptotic cells or apoptotic bodies. In conclusion, DBP administration caused structural degeneration in the epididymis and deferens, parallel to dose evaluation and RSV can reverse these changes with its protective effects.
    Indian Journal of Pharmacology 03/2014; 46(1):51-6. DOI:10.4103/0253-7613.125167 · 0.69 Impact Factor
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