Quantitative SUMO-1 modification of a vaccinia virus protein is required for its specific localization and prevents its self-association.

European Molecular Biology Laboratory, Cell Biology and Biophysics Programme, 69117 Heidelberg, Germany.
Molecular Biology of the Cell (Impact Factor: 4.55). 07/2005; 16(6):2822-35. DOI: 10.1091/mbc.E04-11-1005
Source: PubMed

ABSTRACT Vaccinia virus (VV), the prototype member of the Poxviridae, a family of large DNA viruses, carries out DNA replication in specialized cytoplasmic sites that are enclosed by the rough endoplasmic reticulum (ER). We show that the VV gene product of A40R is quantitatively modified by SUMO-1, which is required for its localization to the ER-enclosed replication sites. Expression of A40R lacking SUMO-1 induced the formation of rod-shaped cytoplasmic aggregates. The latter likely consisted of polymers of nonsumoylated protein, because unmodified A40R interacted with itself, but not with the SUMO-1-conjugated protein. Using a bacterial sumoylation system, we furthermore show that unmodified A40R is mostly insoluble, whereas the modified form is completely soluble. By electron microscopy, the A40R rods seen in cells were associated with the cytosolic side of the ER and induced the apposition of several ER cisternae. A40R is the first example of a poxvirus protein to acquire SUMO-1. Its quantitative SUMO-1 modification is required for its proper localization to the viral "mini-nuclei" and prevents its self-association. The ability of the nonsumoylated A40R to bring ER membranes close together could suggest a role in the fusion of ER cisternae when these coalesce to enclose the VV replication sites.

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    • "This posttranslational modification of proteins with SUMO (sumoylation) is involved in a variety of different cellular pathways, often by regulating protein–protein or protein–DNA interactions (Johnson, 2004; Hay, 2005; Geiss-Friedlander and Melchior, 2007; Wilkinson and Henley, 2010). More recently, increasing evidence suggests that SUMO may also contribute to protein solubility (Palacios et al., 2005; Fei et al., 2006; Mukherjee et al., 2009; Janer et al., 2010). A common feature of sporadic forms of neurodegenerative disease is a decreased solubility of specific disease-associated proteins and, concomitantly, an enhanced pathological propensity to form aggregates. "
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