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Crystallization of mitochondrial respiratory complex II from chicken heart: a membrane-protein complex diffracting to 2.0 A.

University of California, Berkeley, Berkeley, California, United States
Acta Crystallographica Section D Biological Crystallography (Impact Factor: 7.23). 05/2005; 61(Pt 4):380-7. DOI: 10.1107/S0907444905000181
Source: PubMed

ABSTRACT A procedure is presented for preparation of diffraction-quality crystals of a vertebrate mitochondrial respiratory complex II. The crystals have the potential to diffract to at least 2.0 A with optimization of post-crystal-growth treatment and cryoprotection. This should allow determination of the structure of this important and medically relevant membrane-protein complex at near-atomic resolution and provide great detail of the mode of binding of substrates and inhibitors at the two substrate-binding sites.

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