Article

cagA vacA alelles and babA2 genotypes of Helicobacter pylori associated with gastric disease in Brazilian adult patients.

Faculdade de Medicina de Marília (FAMEMA), Marília, São Paulo 17519-050, Brazil.
Diagnostic Microbiology and Infectious Disease (impact factor: 2.53). 05/2005; 51(4):231-5. DOI:10.1016/j.diagmicrobio.2004.11.007 pp.231-5
Source: PubMed

ABSTRACT Helicobacter pylori is an important human pathogen that causes chronic gastritis and is associated with development of peptic ulcer disease and gastric malignancies. The vacuolating cytotoxin (vacA), cagA gene, and babA2 gene are important virulence factor involving gastric diseases. Eighty-nine Helicobacter pylori-positive gastric biopsies were analyzed by polymerase chain reaction and Southern blotting for H. pylori detection and genotyping with primer pairs from each virulence gene. Fifty-three strains (59%) were common vacA genotype s1/m1, and only 14 (16%) were s2/m2, 12% of strains was found to have multiple infection. The cagA presence was detected in 48% (43 strains) and babA2 gene was detected in 44% of our H. pylori strains. We observed high percentage of s1/m1 strains with chronic gastritis and peptic ulcer and a significant correlation between cagA presence with the s1 allele and babA2 gene with chronic gastritis.

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    Article: Infection by Helicobacter pylori expressing the BabA adhesin is influenced by the secretor phenotype.
    M Azevedo, S Eriksson, N Mendes, J Serpa, C Figueiredo, L P Resende, N Ruvoën-Clouet, R Haas, T Borén, J Le Pendu, L David
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    ABSTRACT: Helicobacter pylori (Hp) infects half the world's population and causes diverse gastric lesions, from gastritis to gastric cancer. Our aim was to evaluate the significance of secretor and Lewis status in infection and in vitro adherence by Hp expressing BabA adhesin. We enrolled 304 Hp-infected individuals from Northern Portugal. Gastric biopsies, blood and saliva were collected. Polymerase chain reaction (PCR) and immunofluorescence were used to detect BabA+ Hp in gastric biopsies. In vitro adherence by a BabA expressing Hp strain to gastric biopsies was performed. Secretor status was identified by Ulex, a lectin that recognizes secretor-dependent glycan structures in saliva and in gastric mucosa, and by Lewis(a/b) antibodies, and indirectly by identification of an inactivating mutation in the FUT2 gene (G428A). BabA status of infecting Hp was associated with CagA and VacAs1 (p < 0.05), intercellular localization of Hp (p < 0.01) and the presence of intestinal metaplasia (p < 0.05) and degenerative alterations (p < 0.005) in the biopsies. BabA was associated (p < 0.05) with Ulex staining of gastric biopsies and, although not significantly, to absence of homozygosity for FUT2 G428A inactivating polymorphism. In vitro Hp adherence was higher in cases wild-type or heterozygous for FUT2 G428A mutation (p < 0.0001), cases staining for Ulex (p < 0.0001) and a(-)b+ and a(-)b(-) secretor phenotypes (p < 0.001). In conclusion, BabA+ Hp infection/adhesion is secretor-dependent and associated with the severity of gastric lesions.
    The Journal of Pathology 07/2008; 215(3):308-16. · 6.32 Impact Factor
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Keywords

43 strains
 
cagA gene
 
cagA presence
 
gastric diseases
 
gastric malignancies
 
H. pylori detection
 
Helicobacter pylori
 
Helicobacter pylori-positive gastric biopsies
 
human pathogen
 
multiple infection
 
peptic ulcer
 
peptic ulcer disease
 
polymerase chain reaction
 
s1/m1 strains
 
significant correlation
 
Southern blotting
 
vacuolating cytotoxin
 
virulence factor
 
virulence gene
 

Luciano Lobo Gatti