Article

Anti-diabetic activity of the semi-purified fractions of Averrhoa bilimbi in high fat diet fed-streptozotocin-induced diabetic rats.

Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore.
Life Sciences (impact factor: 2.53). 04/2005; 76(24):2827-39. DOI:10.1016/j.lfs.2004.10.051 pp.2827-39
Source: PubMed

ABSTRACT The present study was designed to investigate the hypoglycemic and hypolipidemic activities of the semi-purified fractions of an ethanolic leaf extract of Averrhoa bilimbi (ABe) in high fat diet (HFD)-streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats aged 10 weeks (200-250 g) were fed with a high fat diet obtained from Glen Forrest stock feeders (Western Australia) for 2 weeks prior to intraperitoneal injection with streptozotocin (STZ, 50 mg/kg). The leaves of A.bilimbi were exhaustively extracted with 80% ethanol, concentrated at 40 degrees C using a rotavapor and partitioned successively with butanol, ethylacetate and hexane to get aqueous (AF), butanol (BuF), ethylacetate (EF), and hexane fractions (HF). The fractions were freeze-dried to obtain powders of each. To investigate the effect of long term administration of the hypoglycemic fractions, diabetic animals were treated with vehicle (distilled water), AF (125 mg/kg), or BuF (125 mg/kg), twice a day for 14 days. The long term administration of AF and BuF at a dose of 125 mg/kg significantly (P < 0.05) lowered blood glucose and triglyceride concentrations when compared to the vehicle. The hepatic glycogen content was significantly higher (P < 0.05) in AF-treated rats when compared to diabetic control, however no change was found in the BuF-treated rats. Moreover, AF as well as BuF did not cause any significant change in the total cholesterol and HDL-cholesterol. There was also no difference in liver thiobarbituric acid reactive substances (TBARS) and cytochrome P450 values between AF, BuF and vehicle-treated control rats. In conclusion, the results indicate that AF is more potent than BuF in the amelioration of hyperglycemia and hyperlipidemia in HFD fed-STZ diabetic rats. Hence, AF is a potential source for the isolation of active principle(s) for oral anti-diabetic therapy.

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Keywords

2 weeks
 
40 degrees C
 
Averrhoa bilimbi
 
cytochrome P450 values
 
diabetic animals
 
diabetic control
 
ethanolic leaf
 
Glen Forrest stock feeders
 
hexane fractions
 
HFD fed-STZ diabetic rats
 
HFD)-streptozotocin
 
hypoglycemic fractions
 
hypolipidemic activities
 
intraperitoneal injection
 
liver thiobarbituric acid reactive substances
 
oral anti-diabetic therapy
 
semi-purified fractions
 
STZ)-induced diabetic rats
 
triglyceride concentrations
 
vehicle-treated control rats
 

Benny Kwong-Huat Tan