Identification of genetic polymorphisms through comparative DNA sequence analysis on the K-ras gene: Implications for lung tumor susceptibility

Department of Surgery and The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Experimental Lung Research (Impact Factor: 1.41). 04/2005; 31(2):165-77. DOI: 10.1080/01902140490495543
Source: PubMed


In the present study, the authors performed a comparative sequence analysis of the K-ras gene. By comparing sequences from different mouse inbred strains, the authors have identified new nucleotide polymorphisms in the noncoding regions of mouse K-ras gene. They have also identified noncoding DNA segments evolutionarily conserved among the human, mouse, and rat. Computational analysis for transcription factor binding sites suggests that these polymorphic and conserved DNA sequences harbor potential cis-regulatory elements, which may contribute to the transcriptional regulation of the K-ras gene. Further studies on these potential regulatory sites may help to elucidate the fundamental mechanism underlying allele-specific activation and expression of K-ras gene in hybrid mouse lung tumors, which determines lung tumor susceptibility in mice.

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    • "Studies employing computational analysis of cancerrelated sequence data have focused mainly on modelling and predicting the effect of mutations, usually at the level of individual nucleotides or codons in a single proto-oncogene or family of proto-oncogenes ( Wang et al. 2005). At the protein sequence level, bioinformatics and proteomics methods have been employed mainly to identify biomarkers for various types of cancers (Cho 2007). "
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