Article

Malondialdehyde, glutathione, glutathione peroxidase and homocysteine levels in type 2 diabetic patients with and without microalbuminuria.

Department of Clinical Biochemistry, Faculty of Medicine, University of Kocaeli, Derince-41900 Kocaeli, Turkey.
Annals of Clinical Biochemistry (impact factor: 2.17). 03/2005; 42(Pt 2):99-104. DOI:10.1258/0004563053492838 pp.99-104
Source: PubMed

ABSTRACT High levels of homocysteine and oxidative stress are known to be associated with premature vascular disease in type 2 diabetes mellitus (DM). The aim of this study was to estimate homocysteine levels and oxidant-antioxidant status and to determine the relationship between them in type 2 diabetic patients with and without microalbuminuria.
Fasting blood samples were obtained from 48 diabetic patients (17 with and 31 without microalbuminuria) and 20 healthy subjects. Serum total homocysteine (tHcy), plasma malondialdehyde (MDA) erythrocyte glutathione (GSH) and glutathione peroxidase (GPx) activity were measured in these patients and the results were compared with those of controls who were chosen among healthy subjects.
MDA levels were found to be significantly lower and GSH levels and GPx activities were found to be significantly higher in control subjects when compared with patients with and without microalbuminuria (MDA: P < 0.0001, P < 0.0001; GSH: P < 0.0001, P < 0.0001; GPx: P < 0.0001, P < 0.001, respectively). MDA levels were found to be significantly higher in patients with microalbuminuria compared with patients without microalbuminuria (P < 0.0001), while similarly GSH levels were found to be significantly lower in patients with microalbuminuria (P < 0.0001). Although there were no significant differences with respect to tHcy levels and GPx activities between the microalbuminuric and normoalbuminuric patients (P > 0.05), there was a significant difference with respect to tHcy levels between healthy controls and patients with microalbuminuria (P < 0.05). The serum levels of tHcy correlated best with plasma MDA and erythrocyte GSH concentrations in all diabetic patients (r = 0.549, P < 0.0001; r = -0.385, P<0.01).
Decreased antioxidant levels, increased lipid peroxidation and increased tHcy levels were observed in patients with microalbuminuria. These changes may contribute to vascular disease, which is particularly prevalent in type 2 DM patients with microalbuminuria.

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Keywords

20 healthy subjects
 
48 diabetic patients
 
Decreased antioxidant levels
 
diabetic patients
 
erythrocyte GSH concentrations
 
estimate homocysteine levels
 
Fasting blood samples
 
glutathione peroxidase
 
GPx activities
 
GSH levels
 
healthy controls
 
healthy subjects
 
normoalbuminuric patients
 
oxidant-antioxidant status
 
serum levels
 
Serum total homocysteine
 
tHcy levels
 
type 2 diabetes mellitus
 
type 2 diabetic patients
 
type 2 DM patients