Article

CpG oligonucleotides induce strong humoral but only weak CD4+ T cell responses to protein antigens in rhesus macaques in vivo.

Department of Internal Medicine, Division of Clinical Pharmacology, Ludwig-Maximilians-University of Munich, Germany.
Vaccine (impact factor: 3.77). 06/2005; 23(25):3310-7. DOI:10.1016/j.vaccine.2005.01.077 pp.3310-7
Source: PubMed

ABSTRACT Oligonucleotides containing CpG motifs (CpG ODN) are strong adjuvants for humoral immune responses but data on cellular immune responses in primates are scarce. Rhesus macaque blood contained similar numbers of plasmacytoid dendritic cells and B cells, the key sensors of CpG ODN, as human blood, and these cells were activated by CpG-A and CpG-B in vitro. In vivo, both ODNs induced equal plasma levels of interferon-inducible protein 10 and similarly enhanced antibody responses following i.m. injections of the ODNs, protein antigen, and aluminium hydroxide into rhesus macaques, whereas antigen-specific CD4(+) T cell responses were only slightly increased by CpG ODN.

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    Article: CpG oligodinucleotides induce strong humoral and cellular responses to swine streptococcic septicemia vaccine in piglets in vivo.
    Zhang Linghua, Guo Yong, Tian Xingshan, Zhou Fengzhen
    [show abstract] [hide abstract]
    ABSTRACT: Oligodinucleotides containing CpG motifs (CpG ODN) are strong adjuvants for immune responses, particularly in mice, but data on humoral and cellular immune responses in piglets are scarce. In this report, using the swine streptococcus as model bacteria, CpG ODN was used as immunoadjuvants to enhance the immune responses of the piglets to swine streptococcic septicemia killed vaccine (SSSK vaccine). The titre of specific antibodies to SSSK vaccine, the proliferation of lymphocytes, SSSK-specific interferon-gamma(IFN-gamma) and IL-6, the expression of major histocompatibility complex class II (MHC-II) and CD14 of peripheral blood mononuclear cells (PBMCs) were examined to identify the immune responses of the piglets. The results were found that the above-mentioned immune responses of the piglets with CpG ODN were significantly stronger than standard immunization protocols. All these data suggested that immunostimulatory CpG ODN was promising immune enhancers for vaccination against SSSK vaccine.
    International Immunopharmacology 04/2006; 6(3):342-50. · 2.38 Impact Factor
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Keywords

antibody responses
 
antigen-specific CD4(+)
 
B cells
 
cellular immune responses
 
CpG-B
 
human blood
 
humoral immune responses
 
i.m. injections
 
interferon-inducible protein 10
 
ODNs induced equal plasma levels
 
plasmacytoid dendritic cells
 
primates
 
protein antigen
 
Rhesus macaque blood
 
similar numbers