Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing

Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
Bioorganic & Medicinal Chemistry Letters (Impact Factor: 2.33). 06/2005; 15(10):2447-51. DOI: 10.1016/j.bmcl.2005.03.079
Source: PubMed

ABSTRACT One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system.


Available from: Dawn Yeo, May 30, 2015