Article

Studies on palauan medicinal herbs. II. Activation of mouse macrophages RAW 264.7 by Ongael, leaves of Phaleria cumingii (Meisn.) F. Vill. and its acylglucosylsterols.

School of Pharmaceutical Sciences, Kinki University, Osaka 577-0818, Japan.
Biological & Pharmaceutical Bulletin (impact factor: 1.66). 06/2005; 28(5):929-33. pp.929-33
Source: PubMed

ABSTRACT The extract of Ongael [leaves of Phaleria cumingii (MEISN.) F. VILL.], a Palauan medicinal herb, enhanced an in vitro phagocytic activity of mouse macrophages RAW 264.7 cells (RAW 264.7). Activity-guided fractionation of the Ongael extract by the in vitro phagocytosis assay using RAW 264.7 led to the isolation of a mixture of acylglucosylsterols (1) as an active constituent along with other inactive constituents, tetracosanol and mangiferin. On the basis of chemical modifications and spectral analyses, the compound 1 was deduced to be a mixture of the known 3-O-(6-O-acyl-beta-D-glucosyl)-beta-sitosterols, the acyl moiety being mainly palmitoyl (57%), oleoyl (12%) and alpha-linolenoyl (12%) with small amount of stearoyl (7%) and linoleoyl (4%).

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Keywords

alpha-linolenoyl
 
chemical modifications
 
compound 1
 
inactive constituents
 
linoleoyl
 
mouse macrophages RAW 264.7 cells
 
Ongael
 
Ongael [leaves
 
Palauan medicinal herb
 
Phaleria cumingii
 
RAW 264.7). Activity-guided fractionation
 
spectral analyses
 
vitro phagocytic activity
 
vitro phagocytosis assay
 

Hideaki Matsuda