Polymorphisms in the 5 ' region of the CD14 gene are associated with eczema in young children
ABSTRACT Variants in the CD14 gene (CD14) are hypothesized to be associated with atopic disorders. However, most studies have only investigated one polymorphism in this gene.
We sought to study the association of 5 single nucleotide polymorphisms (SNPs) in the 5' flanking region of CD14 with eczema and serum IgE levels in young children.
We genotyped 5 SNPs in an approximately 6.5-kb region in the 5' region of CD14 in 344 2-year-old white children from 2 birth cohorts in the northeastern United States. We examined the relation of both single SNPs and haplotypes in CD14 with the atopic outcomes.
Two SNPs were significantly associated with eczema. In dominant models adjusted for potential confounders, SNP rs2569193 was associated with significantly decreased risk for eczema (odds ratio [OR] for CT/TT vs CC, 0.5; 95% CI, 0.3-0.8), whereas SNP rs2569190 (also reported as the C-159T) was associated with significantly increased risk for eczema (OR for CT/TT vs CC, 2.3; 95% CI, 1.4-3.8). The CT/TT genotypes of SNP rs2569190 also had higher geometric means of serum IgE than the CC genotype (24.6 vs 15 IU/mL, P = .025). Haplotype analyses provided results similar to those of the single SNP analyses.
Our results contradict previous reports that have found a protective effect of the T allele of SNP rs2569190 (C-159T) against atopic disorders. Nevertheless, these results confirm the importance of polymorphisms in CD14 in the development of atopy, and future studies of this gene region will need to account for linkage disequilibrium and environmental exposures unique to the study population.
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ABSTRACT: Background Although cesarean delivery and prenatal exposure to antibiotics are likely to affect the gut microbiome in infancy, their effect on the development of atopic dermatitis (AD) in infancy is unclear. The influence of individual genotypes on these relationships is also unclear. To evaluate with a prospective birth cohort study whether cesarean section, prenatal exposure to antibiotics, and susceptible genotypes act additively to promote the development of AD in infancy. Methods The Cohort for Childhood of Asthma and Allergic Diseases (COCOA) was selected from the general Korean population. A pediatric allergist assessed 412 infants for the presence of AD at 1 year of age. Their cord blood DNA was subjected to interleukin (IL)-13 (rs20541) and cluster-of-differentiation (CD)14 (rs2569190) genotype analysis. Results The combination of cesarean delivery and prenatal exposure to antibiotics associated significantly and positively with AD (adjusted odds ratio, 5.70; 95% CI, 1.19–27.3). The association between cesarean delivery and AD was significantly modified by parental history of allergic diseases or risk-associated IL-13 (rs20541) and CD14 (rs2569190) genotypes. There was a trend of interaction between IL-13 (rs20541) and delivery mode with respect to the subsequent risk of AD. (P for interaction = 0.039) Infants who were exposed prenatally to antibiotics and were born by cesarean delivery had a lower total microbiota diversity in stool samples at 6 months of age than the control group. As the number of these risk factors increased, the AD risk rose (trend p<0.05). Conclusion Cesarean delivery and prenatal antibiotic exposure may affect the gut microbiota, which may in turn influence the risk of AD in infants. These relationships may be shaped by the genetic predisposition.PLoS ONE 05/2014; 9(5):e96603. DOI:10.1371/journal.pone.0096603 · 3.53 Impact Factor
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ABSTRACT: Films of PVA/PVP blend (50/50) filled with different concentrations of NiCl2 were prepared by casting method. The prepared films were investigated by different techniques. XRD scans demonstrated that the peak intensity at 2θ≈20° decreased and the band width increased with increase in the concentrations of NiCl2 content, which implied decrease in the degree of crystallization and hence causes increase in the amorphous region. UV–vis analysis revealed that the values of the optical band gap are affected with increase in NiCl2 content. This indicates the formation of charge transfer complexes between the polymer blend and the filler. The rise of conductivity is significant with increased concentration of NiCl2 filler; this reveals an increase in degree of amorphosity. AC conductivity (σac) behavior of all the prepared films was investigated over the frequency range 42Hz–5MHz and under different isothermal stabilization in the temperature range 313–393K. It suggests that the hopping mechanism might be playing an important role in the conduction process in high frequency region. The dielectric behavior was analyzed using dielectric permittivity (ε´, ε″) dielectric loss tangent (tanδ) and electric modulus (M″). The decrease in dielectric permittivity was observed with increase in the concentration of NiCl2 filler. This suggests the role of NiCl2 as filler to improve the electrical conductivity of PVA/PVP blend.Physica B Condensed Matter 10/2011; 406(20):3759-3767. DOI:10.1016/j.physb.2010.11.030 · 1.28 Impact Factor
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ABSTRACT: Studying gene-environment interactions may elucidate the complex origins of atopic diseases. The aim of this study was to evaluate the association of CD14 polymorphisms and atopy in Egyptian children and to study whether atopy is influenced by CD14 interaction with tobacco smoke exposure. CD14 -159 C/T and CD14 -550 C/T were genotyped in 500 asthmaic children, 150 allergic rhinitis children and 150 controls. We found that CD14 -159T allele, CD14 -550T allele and CD14 -159T/-550T haplotype were significantly associated with atopic asthma and allergic rhinitis groups. CD14 -159 TT and CD14 -550 TT genotypes associated with elevated IgE levels in children exposed to tobacco smoke. The TT genotype of CD14 -159 C/T and CD14 -550 C/T was associated with higher serum levels of sCD14. The present study indicated that CD14 gene polymorphisms may contribute to susceptibility to atopy in Egyptian children and influenced with tobacco smoke exposure.Cellular Immunology 08/2013; 285(1-2):31-37. DOI:10.1016/j.cellimm.2013.08.001 · 1.87 Impact Factor