Article

Interferon-γ promotes abortion due to Brucella infection in pregnant mice

Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
BMC Microbiology (Impact Factor: 2.98). 02/2005; 5:22. DOI: 10.1186/1471-2180-5-22
Source: PubMed

ABSTRACT The mechanisms of abortion induced by bacterial infection are largely unknown. In the present study, we investigated abortion induced by Brucella abortus, a causative agent of brucellosis and facultative intracellular pathogen, in a mouse model.
High rates of abortion were observed for bacterial infection on day 4.5 of gestation, but not for other days. Regardless of whether fetuses were aborted or stayed alive, the transmission of bacteria into the fetus and bacterial replication in the placenta were observed. There was a higher degree of bacterial colonization in the placenta than in other organs and many bacteria were detected in trophoblast giant cells in the placenta. Intracellular growth-defective virB4 mutant and attenuated vaccine strain S19 did not induce abortion. In the case of abortion, around day 7.5 of gestation (period of placental development), transient induction of IFN-gamma production was observed for infection by the wild type strain, but not by the virB4 mutant and S19. Neutralization of IFN-gamma, whose production was induced by infection with B. abortus, served to prevent abortion.
These results indicate that abortion induced by B. abortus infection is a result of transient IFN-gamma production during the period of placental development.

0 Followers
 · 
222 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The human body is bordered by the skin and mucosa, which are the cellular barriers that define the frontier between the internal milieu and the external nonsterile environment. Additional cellular barriers, such as the placental and the blood-brain barriers, define protected niches within the host. In addition to their physiological roles, these host barriers provide both physical and immune defense against microbial infection. Yet, many pathogens have evolved elaborated mechanisms to target this line of defense, resulting in a microbial invasion of cells constitutive of host barriers, disruption of barrier integrity, and systemic dissemination and invasion of deeper tissues. Here we review representative examples of microbial interactions with human barriers, including the intestinal, placental, and blood-brain barriers, and discuss how these microbes adhere to, invade, breach, or compromise these barriers.
    Cold Spring Harbor Perspectives in Medicine 07/2013; 3(7). DOI:10.1101/cshperspect.a010090
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The JAR choriocarcinoma cell line share many of the characteristics of early placental trophoblast cells including the invasion properties. Matrix metallo-proteinases (MMPs), the main actors of matrix proteolysis, are involved in normal invasion as well as in the invasive character of tumor cells and the metastase formation. Tumor necrosis factor-α (TNF-α) is present in the placental environment and TNF-α levels are elevated in some placental pathologies. In the present work, we addressed whether TNF-α is a modulator of JAR cell MMP secretion. Following TNF-α stimulation, zymographic analysis showed the increased secretion of the active form of MMP-2 and to a lesser extent proMMP-2 and MMP-9. In addition, MMP-2 gene expression only increased slightly whereas MMP-9 and TIMP-1 transcripts were undetectable. This suggests that TNF-α may modulate the secretion of MMPs independently of MMP gene expression control.
    Advances in Bioscience and Biotechnology 02/2012; 3(1):51-56. DOI:10.4236/abb.2012.31009
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Brucellosis is one of the most important zoonotic diseases worldwide, resulting in serious economic losses and public health issues. It is caused by intracellular Gram-negative bacteria of the genus Brucella, which are responsible for a debilitating disease in humans and a chronic infection in domestic animals. The present article considers the pathogenesis of Brucella spp., with the goal to cover clinical aspects of the disease in the different mammalian species along with the target cells used by this pathogen to survive inside the host. Additionally, important molecular mechanisms used by Brucella to invade and persist inside the hosts target cells are also discussed.
    The Open Veterinary Science Journal 05/2010; 4(1). DOI:10.2174/1874318801004010109

Preview (2 Sources)

Download
4 Downloads
Available from