Fabrication of Porous Participate for the Scaffold by Applying Solution Spraying Method

Division of Dental Anesthesiology, Graduate School of Dentistry, Osaka University, Osaka, Japan.
Dental Materials Journal (Impact Factor: 0.97). 04/2005; 24(1):76-82. DOI: 10.4012/dmj.24.76
Source: PubMed


A simple and novel method--in the form of solution spraying--was developed to fabricate biodegradable, porous poly(L-lactic acid) (PLLA) particulates for scaffold. PLLA pellets were dissolved in an organic solvent. Then, 5 % PLLA-dioxane solution was sprayed using an air-assisted atomizer with a nozzle diameter of 2.5 mm at an air flow rate of 15 L/min. After the sprayed solution solidified in liquid nitrogen, spherical particulates with median diameter of 225microm were obtained. Morphology of sprayed products could be altered by varying the fabrication conditions. When nozzle diameter was reduced to 1.5 mm, sprayed products became fibrous. When the concentration of PLLA-dioxane solution was increased, the diameter of particulates increased too. On the other hand, when air flow rate was increased, the diameter of particulates decreased. Likewise, solidification conditions also affected the morphology of sprayed products, such that they were either thin film-like or in particulate form. Based on the results of the present study, we concluded that PLLA particulates of varying morphologies could be obtained by adjusting the fabrication conditions.

1 Follower
5 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study investigated the influence of atmospheric pressure plasma treatment on the surface properties and cell response of poly(L-lactide) (PLLA) samples. The samples were analyzed by means of X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), micro- and nanosurface roughness, water contact angle, and zeta potential. Furthermore, cell adhesion assay and cell proliferation assay on the samples were carried out using MC3T3-E1 cells. Plasma treatment significantly increased the oxygen content of the samples and decreased the contact angle and zeta potential of the samples, resulting in hydrophilic surfaces. Further, plasma treatment of the samples also enhanced the number and growth of adhering MC3T3-E1 cells. These results therefore indicate that plasma treatment is effective for surface modification and cell responses.
    Dental Materials Journal 10/2006; 25(3):560-5. DOI:10.4012/dmj.25.560 · 0.97 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: It is well established that the pore size and distribution affect the rate of cell migration and the extent of extracellular matrix formation. The objective of this work was to develop a process for fabrication of biodegradable and shape-specific polymeric scaffolds with well-defined pore geometry, functionalized with covalently attached bioactive peptides, for applications in tissue regeneration. We have used the Fused Deposition Modeling (FDM) RP technology to fabricate degradable and functional scaffolds with well-defined pore geometry. Computer aided design (CAD) using SolidWorks was used to create models of the cubic orthogonal geometry. The models were used to create the machine codes necessary to build the scaffolds with FDM with wax as the build material. A novel biodegradable in-situ crosslinkable macromer, poly(lactide-co-glycolide fumarate) or PLGF, mixed with reactive functional peptides was infused in the scaffold and allowed to crosslink. The scaffold was then immersed in a hydrocarbon solvent to remove the wax, leaving just the PLGF behind as the support material dissolved. The pore morphology of the PLGF scaffold was imaged with micro-computed tomography and scanning electron microscopy. Cellular function in the PLFG scaffolds with well-defined pore geometry was studied with bone marrow stromal cells isolated from rats. Results demonstrate that the scaffolds support homogeneous formation of mineralized tissue.
    ASME 2007 International Manufacturing Science and Engineering Conference; 01/2007
  • [Show abstract] [Hide abstract]
    ABSTRACT: Injectable polycaprolactone (PCL) porous beads were fabricated for use as cell carriers by a novel isolated particle-melting method (for nonporous beads) and the following melt-molding particulate-leaching method (for porous beads). The prepared beads showed highly porous and uniform pore structures with almost the same surface and interior porosities (porosity, over 90%). The PCL porous beads (bead size, 400-550 microm) with different pore sizes (25-50 and 50-100 microm) were compared for their in vitro cell (human chondrocyte) growth behavior with the nonporous beads. The porous beads showed higher cell seeding density and growth than the nonporous beads. The pore size effect between the porous beads was not significant up to 7 days, but after that time the beads with pore sizes of 50-100 microm showed significantly higher cell growth than those of 25-50 microm. To evaluate the tissue compatibility of the PCL porous beads, the beads were dispersed, uniformly, in cold Pluronic F127 solution and injected into hairless mice, subcutaneously, in the gel state of Pluronic F127 at room temperature, leading to the homogeneous bead delivery. The histological findings confirmed that the PCL porous beads in Pluronic F127 gel are biocompatible: surrounding tissues gradually infiltrated into the porous beads for up to 4 weeks with little inflammatory response. The PCL porous beads with highly porous and uniform pore structures fabricated in this study can be widely applicable as cell carriers.
    Journal of Biomedical Materials Research Part B Applied Biomaterials 08/2009; 90(2):521-30. DOI:10.1002/jbm.b.31313 · 2.76 Impact Factor
Show more