Article

Multicenter evaluation of the nitrate reductase assay for drug resistance detection of Mycobacterium tuberculosis

Universidad Peruana Cayetano Heredia, Λίμα, Lima, Peru
Journal of Microbiological Methods (Impact Factor: 2.1). 12/2005; 63(2):145-50. DOI: 10.1016/j.mimet.2005.03.004
Source: PubMed

ABSTRACT The performance of the nitrate reductase assay was evaluated in a multicenter laboratory study to detect resistance of Mycobacterium tuberculosis to the first-line anti-tuberculosis drugs rifampicin, isoniazid, ethambutol and streptomycin using a set of coded isolates. Compared with the gold standard proportion method on Löwenstein-Jensen medium, the assay was highly accurate in detecting resistance to rifampicin, isoniazid and ethambutol with an accuracy of 98%, 96.6% and 97.9%, respectively. For streptomycin, discrepant results were obtained with an overall accuracy of 85.3%. The assay proved easy to be implemented in countries with limited laboratory facilities.

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    ABSTRACT: MS. Utility of nitrate reductase assay for detection of multidrug-resistant Mycobacterium tuberculosis in a low resource setting. Biomédica. 2011;31(2). El Comité Editorial de Biomédica ya aprobó para publicación este manuscrito, teniendo en cuenta los conceptos de los pares académicos que lo evaluaron. Se publica anticipadamente en versión pdf en forma provisional con base en la última versión electrónica del manuscrito pero sin que aún haya sido diagramado ni se le haya hecho la corrección de estilo. Siéntase libre de descargar, usar, distribuir y citar esta versión preliminar tal y como lo indicamos pero, por favor, recuerde que la versión impresa final y en formato pdf pueden ser diferentes.
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