Article

Enhancing of measles virus infection by magnetofection.

Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
Journal of Virological Methods (impact factor: 2.01). 10/2005; 128(1-2):61-6. DOI:10.1016/j.jviromet.2005.04.003 pp.61-6
Source: PubMed

ABSTRACT Magnetofection is a viral and non-viral gene delivery method using polyethyleneimine-conjugated super-paramagnetic nanoparticle under a magnetic field. Previous studies have indicated that magnetofection enhanced the infection of adenoviruses and retroviruses. It is shown that magnetofection enhances the infection of measles virus, a paramyxovirus. When cells expressing a measles virus receptor human SLAM were infected with a measles virus that encodes green fluorescent protein gene, magnetofection enhanced measles virus infection by 30- to 70-fold. The infection of SLAM-negative cells with measles virus was also enhanced by magnetofection, but to a lesser extent. These results indicate that magnetofection could be useful for isolation of measles virus from clinical specimens.

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    Article: Simple, efficient, and reproducible gene transfection of mouse embryonic stem cells by magnetofection.
    Chang Hyun Lee, Eun Young Kim, Kilsoo Jeon, Jin Cheol Tae, Keum Sil Lee, Yeon Ok Kim, Mi-Young Jeong, Cheol-Won Yun, Dong Kee Jeong, Somi K Cho, Jae Hoon Kim, Hyo Yeon Lee, Key Zung Riu, Ssang Goo Cho, Se Pill Park
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    Stem Cells and Development 02/2008; 17(1):133-41. · 4.46 Impact Factor
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    Article: Enhancement of the efficiency of non-viral gene delivery by application of pulsed magnetic field.
    Sarah W Kamau, Paul O Hassa, Benedikt Steitz, Alke Petri-Fink, Heinrich Hofmann, Margarethe Hofmann-Amtenbrink, Brigitte von Rechenberg, Michael O Hottiger
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    ABSTRACT: New approaches to increase the efficiency of non-viral gene delivery are still required. Here we report a simple approach that enhances gene delivery using permanent and pulsating magnetic fields. DNA plasmids and novel DNA fragments (PCR products) containing sequence encoding for green fluorescent protein were coupled to polyethylenimine coated superparamagnetic nanoparticles (SPIONs). The complexes were added to cells that were subsequently exposed to permanent and pulsating magnetic fields. Presence of these magnetic fields significantly increased the transfection efficiency 40 times more than in cells not exposed to the magnetic field. The transfection efficiency was highest when the nanoparticles were sedimented on the permanent magnet before the application of the pulsating field, both for small (50 nm) and large (200-250 nm) nanoparticles. The highly efficient gene transfer already within 5 min shows that this technique is a powerful tool for future in vivo studies, where rapid gene delivery is required before systemic clearance or filtration of the gene vectors occurs.
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Keywords

encodes green fluorescent protein gene
 
lesser extent
 
magnetic field
 
magnetofection
 
magnetofection enhances
 
measles virus
 
measles virus infection
 
measles virus receptor human SLAM
 
non-viral gene delivery method
 
polyethyleneimine-conjugated super-paramagnetic nanoparticle
 
Previous studies
 
SLAM-negative cells
 
viral
 

Shin-Ichi Kadota