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Enhancing of measles virus infection by magnetofection

Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
Journal of Virological Methods (Impact Factor: 1.88). 10/2005; 128(1-2):61-6. DOI: 10.1016/j.jviromet.2005.04.003
Source: PubMed

ABSTRACT Magnetofection is a viral and non-viral gene delivery method using polyethyleneimine-conjugated super-paramagnetic nanoparticle under a magnetic field. Previous studies have indicated that magnetofection enhanced the infection of adenoviruses and retroviruses. It is shown that magnetofection enhances the infection of measles virus, a paramyxovirus. When cells expressing a measles virus receptor human SLAM were infected with a measles virus that encodes green fluorescent protein gene, magnetofection enhanced measles virus infection by 30- to 70-fold. The infection of SLAM-negative cells with measles virus was also enhanced by magnetofection, but to a lesser extent. These results indicate that magnetofection could be useful for isolation of measles virus from clinical specimens.

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    • "3. CombiMag has been used successfully with plasmid DNA, antisense oligonucleotides, mRNA, siRNA, and viruses. This reagent is designed that it can combine with any commercially available transfection reagent such as cationic polymers and lipids (Kadota et al.,2005). 4. SilenceMag provides more efficient method for delivery of siRNA even at low doses. "
    Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications, 04/2011; , ISBN: 978-953-307-197-8
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    • "They form stable complexes and DNA is protected from degradation (Hildebrandt et al., 2003). However, SPION can be used with viral vectors like paramyxoviruses (Kadota et al., 2005). In the presence of static magnets, the coupled magnetic polyplex nanoparticles display a transfection efficiency of reporter genes comparable with conventional nonviral transfection systems. "
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    ABSTRACT: Embryonic stem (ES) cells are recognized as an excellent cell culture model for studying develop- mental mechanisms and their therapeutic modulations. The aim of this work was to define whether using magnetofection was an efficient way to manipulate stem cells genetically without adversely af- fecting their proliferation or self-renewal capacity. We compared our magnetofection results to those of a conservative method using FuGENE 6. Using enhanced green fluorescent protein (eGFP) as a reporter gene in D3 mouse ES (mES) cells, we found that magnetofection gave a significantly higher efficiency (45%) of gene delivery in stem cells than did the FuGENE 6 method (15%), whereas both demonstrated efficienct transfection in NIH-3T3 cells (60%). Although the transfected D3 (D3-eGFP) mES cells had undergone a large number of passages (� 50), a high percentage of cells retained ES markers such as Oct-4 and stage-specific embryonic antigen-1 (SSEA-1). They also retained the abil- ity to form embryoid bodies and differentiated in vitro into cells of the three germ layers. eGFP ex- pression was sustained during stem cell proliferation and differentiation. This is the first transfec- tion report using magnetofection in ES cells. On the basis of our results, we conclude that magnetofection is an efficient and reliable method for the introduction of foreign DNA into mouse ES cells and may become the method of choice.
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