Article

Protein microarray for profiling antibody responses to Yersinia pestis live vaccine.

Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Infection and Immunity (Impact Factor: 4.07). 07/2005; 73(6):3734-9. DOI: 10.1128/IAI.73.6.3734-3739.2005
Source: PubMed

ABSTRACT A protein microarray representing 149 Yersinia pestis proteins was developed to profile antibody responses in EV76-immunized rabbits. Antibodies to 50 proteins were detected. There are 11 proteins besides F1 and V antigens to which the predominant antibody response occurred, and these proteins show promise for further evaluation as candidates for subunit vaccines and/or diagnostic antigens.

1 Bookmark
 · 
110 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objectives Over the past decade, the introduction of techniques of molecular biology and the automation have undergone important changes in the practice of clinical microbiological laboratories. Methods Commonly, physician prescriptions are etiology driven for emerging infectious agent. The improving in test standardization and rapidity and the best communication between physician and clinical microbiologist lead to profound changes in microbiology laboratories. Results and conclusion The present review summarizes the recent development in multiplexed microbiological technology adapted to syndrome driven diagnosis, including sampling strategy, DNA microarray and antigenic microarray.
    Antibiotiques 01/2009; 11(1):37-48.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Over the last years several solutions, based on either a directly modulated DFB laser or an external modulator, have been used in AM CATV systems. The individual effects of clipping and chirping distortions on the performances of CATV systems using directly modulated lasers have been analysed in different papers in terms of CTB, CSO and NLD. The aim of this paper is to analyse and compare the different modulation schemes taking simultaneously into account all the effects influencing the transmission performance. Here we consider four different cases. The first refers to directly modulated DFB lasers (DM-DFB), the other three relate to external modulation using a Mach-Zehnder modulator (MZ). In particular these cases refer to conventional MZ modulators (C-MZ), to linearized modulators (L-MZ) and to a predistorted modulation scheme (P-MZ). Nonlinear distortion limits the performance of CATV distribution systems. In particular, besides clipping, the direct modulation of lasers is significantly affected by chirping induced distortions. A reduction of the chirping/dispersion effect, obtained either by using dispersion shifted fiber, or reducing the overall lengths, can rend the direct modulation of the laser still applicable. In any case the predistorted technique appears to be the best solution concerning the transmission performance
    01/1996;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The development of new vaccines would be greatly facilitated by having effective methods to predict vaccine performance. Such methods could also be helpful in monitoring individual vaccine responses to existing vaccines. We have developed "immunosignaturing" as a simple, comprehensive, chip-based method to display the antibody diversity in an individual on peptide arrays. Here we examined whether this technology could be used to develop correlates for predicting vaccine effectiveness. By using a mouse influenza infection, we show that the immunosignaturing of a natural infection can be used to discriminate a protective from nonprotective vaccine. Further, we demonstrate that an immunosignature can determine which mice receiving the same vaccine will survive. Finally, we show that the peptides comprising the correlate signatures of protection can be used to identify possible epitopes in the influenza virus proteome that are correlates of protection.
    Proceedings of the National Academy of Sciences 10/2013; · 9.81 Impact Factor

Full-text (2 Sources)

Download
20 Downloads
Available from
May 20, 2014