Protein Microarray for Profiling Antibody Responses to Yersinia pestis Live Vaccine

Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Infection and Immunity (Impact Factor: 3.73). 07/2005; 73(6):3734-9. DOI: 10.1128/IAI.73.6.3734-3739.2005
Source: PubMed


A protein microarray representing 149 Yersinia pestis proteins was developed to profile antibody responses in EV76-immunized rabbits. Antibodies to 50 proteins were detected.
There are 11 proteins besides F1 and V antigens to which the predominant antibody response occurred, and these proteins show
promise for further evaluation as candidates for subunit vaccines and/or diagnostic antigens.

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    • "Rucker and coworkers have successfully developed antibody-based microarray techniques for the multiplexed detection of cholera toxin β-subunit, diphtheria toxin, anthrax lethal factor, and protective antigen, Staphyloccus aureus enterotoxin B, and tetanus toxin C fragment from spiked samples [57]. Li et al. used a protein microarray spotting with 149 Y. pestis proteins to profile antibody responses to a Y. pestis live vaccine [58]. With the continuing innovation for this technology, some limitations need to be addressed, as well. "
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    ABSTRACT: Yersinia enterocolitica is an important zoonotic pathogen that can cause yersiniosis in humans and animals. Food has been suggested to be the main source of yersiniosis. It is critical for the researchers to be able to detect Yersinia or any other foodborne pathogen with increased sensitivity and specificity, as well as in real-time, in the case of a foodborne disease outbreak. Conventional detection methods are known to be labor intensive, time consuming, or expensive. On the other hand, more sensitive molecular-based detection methods like next generation sequencing, microarray, and many others are capable of providing faster results. DNA testing is now possible on a single molecule, and high-throughput analysis allows multiple detection reactions to be performed at once, thus allowing a range of characteristics to be rapidly and simultaneously determined. Despite better detection efficiencies, results derived using molecular biology methods can be affected by the various food matrixes. With the improvements in sample preparation, data analysis, and testing procedures, molecular detection techniques will likely continue to simplify and increase the speed of detection while simultaneously improving the sensitivity and specificity for tracking pathogens in food matrices.
    10/2011; 2011(2):310135. DOI:10.4061/2011/310135
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    • "These proteins were considered to be immunogens for humans because they can induce humoral responses during Y. pestis infection. Except for 10 proteins that were discovered to be immunogens in previous studies (Anderson et al. 1996; Andrews et al. 1996; Benner et al. 1999; Leary et al. 1999; Li et al. 2005b, 2008; Tanabe et al. 2006), the immunogenicities of the other 11 proteins ( "
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    ABSTRACT: Yersinia pestis is a bacterium that is transmitted between fleas, which have a body temperature of 26 °C, and mammalian hosts, which have a body temperature of 37 °C. To adapt to the temperature shift, phenotype variations, including virulence, occur. In this study, an antigen microarray including 218 proteins of Y. pestis was used to evaluate antibody responses in a pooled plague serum that was unadsorbed, adsorbed by Y. pestis cultivated at 26 °C, or adsorbed by Y. pestis cultivated at 26 and 37 °C to identify protein expression changes during the temperature shift. We identified 12 proteins as being expressed at 37 °C but not at 26 °C, or expressed at significantly higher levels at 37 °C than at 26 °C. The antibodies against 7 proteins in the serum adsorbed by Y. pestis cultivated at 26 and 37 °C remained positive, suggesting that they were not expressed on the surface of Y. pestis in LB broth in vitro or specifically expressed in vivo. This study proved that protein microarray and antibody profiling comprise a promising technique for monitoring gene expression at the protein level and for better understanding pathogenicity, to find new vaccine targets against plague.
    Canadian Journal of Microbiology 04/2011; 57(4):287-94. DOI:10.1139/w11-007 · 1.22 Impact Factor
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    • "A high degree of cross-reactivity in the antibody response to Burkholderia and related bacteria was also previously reported [29]. Further, a protein microarray consisting of 4% of Y. pestis proteins was used by Li et al. to profile antibody responses to live plague vaccine in rabbits [30], sentinel animals [31], and plague patients [32]. Predominant responses were observed for 11 proteins in addition to F1 and V antigens in rabbits. "
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    ABSTRACT: Protein microarrays are powerful tools that are widely used in systems biology research. For infectious diseases, proteome microarrays assembled from proteins of pathogens will play an increasingly important role in discovery of diagnostic markers, vaccines, and therapeutics. Distinct formats of protein microarrays have been developed for different applications, including abundance-based and function-based methods. Depending on the application, design issues should be considered, such as the need for multiplexing and label or label free detection methods. New developments, challenges, and future demands in infectious disease research will impact the application of protein microarrays for discovery and validation of biomarkers.
    International Journal of Molecular Sciences 12/2010; 11(12):5165-83. DOI:10.3390/ijms11125165 · 2.86 Impact Factor
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