Article

Analytical assessment of MALDI-TOF Imaging Mass Spectrometry on thin histological samples. An insight in proteome investigation.

Centro Studi sull'Invecchiamento (Ce.S.I.), Fondazione Università "G.D'Annunzio", Chieti, Italy.
Clinica Chimica Acta (impact factor: 2.54). 08/2005; 357(2):210-8. DOI:10.1016/j.cccn.2005.03.029 pp.210-8
Source: PubMed

ABSTRACT The development of MALDI Imaging Mass Spectrometry is a promising technique in the investigation of biological molecular repertoire. We have pursued an analytical assessment of this technique in its application to proteome analysis.
A specific statistical method of analysis has been developed to enable data processing in the absence of internal standards, by defining similarity scores.
The investigated linear mode MALDI-TOF set-up allows to obtain data variations comprised within the 30% of variation when assaying tissues samples from the same animal, while the 60% of variation was highlighted in the inter-mice series assaying syngenic animals.
This analytical assessment represents the first step of a process that should validate the utilisation of this technique in the clinical practice.

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Keywords

assaying tissues samples
 
biological molecular repertoire
 
clinical practice
 
data processing
 
first step
 
inter-mice series assaying syngenic animals
 
internal standards
 
investigated linear mode MALDI-TOF set-up
 
MALDI Imaging Mass Spectrometry
 
proteome analysis
 
specific statistical method