Isolation and characterization of multipotent skin-derived precursors from human skin.
ABSTRACT We have previously isolated, expanded, and characterized a multipotent precursor cell from mammalian dermis (termed skin-derived precursors [SKPs]) that can differentiate into both neural and mesodermal progeny. In this study, we report the isolation, expansion, and characterization of a similar precursor cell from neonatal human foreskin tissue. Like their rodent counterparts, human SKPs grew in suspension as spheres in the presence of the mitogens fibroblast growth factor 2 and epidermal growth factor and expressed nestin, fibronectin, vimentin, and characteristic embryonic transcription factors. Human SKPs could be maintained in culture for long periods of time and would still differentiate into neurons, glia, and smooth muscle cells, including cells with the phenotype of peripheral neurons and Schwann cells. Clonal analysis indicated that single SKP cells were multipotent and could give rise to all of these progeny. Moreover, human SKPs apparently derive from an endogenous precursor within human foreskin; a subpopulation of dissociated primary foreskin cells could differentiate into neurons, a cell type never seen in skin, and the initial spheres to develop from skin expressed the same markers and had the same potential as do passaged SKPs. Together, these data indicate that SKPs are an endogenous multipotent precursor cell present in human skin that can be isolated and expanded and differentiate into both neural and mesodermal cell types.
Dataset: dupin 2007
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ABSTRACT: Current stem cell research greatly depends on suitable in vitro-cultivation approaches, enabling expansion, differentiation, cryopreservation or genetic modification of stem cells outside the organism. Particularly regarding neurodegenerative diseases, regeneration of complex injuries or cancer, this already great field of applications is even broadened by in vitro-culture approaches for stem cell-based therapy. Here, 2D-concepts of cultivation focus on cell differentiation or short-time expansion for further transplantation as well as the elucidation of molecular mechanisms of a certain disease for drug targeting. However, latest studies suggest potential beneficial effects of 3D cultivation strategies. In contrast to 2D-culture, the conditions of the endogenous stem cell niche are mirrored more closely, leading to improved cellular viability and differentiation behavior. The use of 3D-cultivated stem cell-products may provide the advantages of direct transplantation, enhanced graft adherence and higher cellular loading densities within the transplant. Thus, together with the increased differentiation potential of stem cells under such conditions, 3D-culture may provide a powerful tool for regenerative medicine. Here, we summarize current 3D-cultivation approaches for adult, embryonic and cancer stem cells, highlighting their potential scientific and clinical impacts.Current Tissue Engineering. 03/2013; 2(1):8-19.
Dataset: p38MAPK inhibition: a new combined approach to reduce neuroblastoma resistance under etoposide treatment