Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells.

Novartis Institutes for BioMedical Research, Basel, Switzerland.
Journal of Biomolecular NMR (Impact Factor: 2.85). 04/2005; 31(4):343-9. DOI: 10.1007/s10858-005-2451-3
Source: PubMed

ABSTRACT This report shows for the first time the efficient uniform isotope labeling of a recombinant protein expressed using Baculovirus-infected insect cells. The recent availability of suitable media for (15)N- and (13)C/(15)N-labeling in insect cells, the high expression of Abl kinase in these labeling media and a suitable labeling protocol made it possible to obtain a (1)H-(15)N-HSQC spectrum for the catalytic domain of Abl kinase of good quality and with label incorporation rates > 90%. The presented isotope labeling method should be applicable also to further proteins where successful expression is restricted to the Baculovirus expression system.

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