Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor

Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, Peking University, Beijing 100871, P.R. China.
Biochimica et Biophysica Acta (Impact Factor: 4.66). 07/2005; 1750(1):14-6. DOI: 10.1016/j.bbapap.2005.03.013
Source: PubMed


The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 A resolution in-house and belongs to space group P2(1) with unit-cell parameters a=64.10 A, b=34.88 A, c=114.72 A, beta=90.49 degrees .

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