The antibacterial activity of honey against coagulase-negative staphylococci.
ABSTRACT Development of antibiotic-resistant strains of coagulase-negative staphylococci has complicated the management of infections associated with the use of invasive medical devices, and innovative treatment and prophylactic options are needed. Honey is increasingly being used to treat infected wounds, but little is known about its effectiveness against coagulase-negative staphylococci. The aim of this study was to determine the minimum active dilution of two standardized, representative honeys for 18 clinical isolates of coagulase-negative staphylococci.
An agar incorporation technique was used to determine the minimum active dilution, with dilution steps of 1% (v/v) honey [or steps of 5% (v/v) of a sugar syrup matching the osmotic effect of honey]. The plates were inoculated with 10 microL spots of cultures of the isolates.
The honeys were inhibitory at dilutions down to 3.6 +/- 0.7% (v/v) for the pasture honey, 3.4 +/- 0.5% (v/v) for the manuka honey and 29.9 +/- 1.9% (v/v) for the sugar syrup.
Typical honeys are about eight times more potent against coagulase-negative staphylococci than if bacterial inhibition were due to their osmolarity alone. Therefore, honey applied to skin at the insertion points of medical devices may have a role in the treatment or prevention of infections by coagulase-negative staphylococci.
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ABSTRACT: Honey is the only insect-derived natural product with therapeutic, traditional, spiritual, nutritional, cosmetic, and industrial value. In addition to having excellent nutritional value, honey is a good source of physiologically active natural compounds, such as polyphenols. Unfortunately, there are very few current research projects investigating the nootropic and neuropharmacological effects of honey, and these are still in their early stages. Raw honey possesses nootropic effects, such as memory-enhancing effects, as well as neuropharmacological activities, such as anxiolytic, antinociceptive, anticonvulsant, and antidepressant activities. Research suggests that the polyphenol constituents of honey can quench biological reactive oxygen species and counter oxidative stress while restoring the cellular antioxidant defense system. Honey polyphenols are also directly involved in apoptotic activities while attenuating microglia-induced neuroinflammation. Honey polyphenols are useful in improving memory deficits and can act at the molecular level. Therefore, the ultimate biochemical impact of honey on specific neurodegenerative diseases, apoptosis, necrosis, neuroinflammation, synaptic plasticity, and behavior-modulating neural circuitry should be evaluated with appropriate mechanistic approaches using biochemical and molecular tools.Evidence-based Complementary and Alternative Medicine 01/2014; · 1.72 Impact Factor
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ABSTRACT: RESUMO Objetivo: Avaliar o impacto do uso de Mupirocina tópica em inserção de cateter venoso central para hemodiálise. Métodos: Revisão Sistemática com Metanálise. Resultados: Após uma criteriosa e extensa busca, foram incluídos três ensaios clínicos que compararam o uso de Mupirocina versus outra intervenção em cateter venoso central para hemodiálise. Conclusão: O estudo apontou que o uso de Mupirocina tópica é eficaz para redução dos episódios de infecções entre os pacientes em hemodiálise, aumentando o tempo de utilização do cateter, além de reduzir significativamente as infecções por S aureus as mais prevalentes nessa população. ABSTRACT Objective: To evaluate the impact of the use of topical Mupirocin on the insertion of central venous catheter for hemodialysis. Methods: This was a systematic review with meta-analysis. Results: After a careful and extensive search, we included three clinical trials that compared the use of Mupirocin versus other intervention in central venous catheter for hemodialysis. Conclusion: The study found that the use of topical Mupirocin is effective in reducing episodes of infection among hemodialysis patients, increasing duration time for catheter, and significantly reducing S aureus infections, which are the most prevalent in this population.Acta Paulista de Enfermagem 01/2012; 25(1). · 0.27 Impact Factor
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ABSTRACT: Honey is rich with complex natural components which could be useful as antibacterial agents or as preservative. Honey contains high concentration of sugars, low amount of water, high osmolality and often dark colour which influence its antibacterial activity. Disc diffusion, well method, micro dilution assay are methods commonly used to determine the antibacterial activity of honey. In this study, microtiter and microbial plate count were included to ascertain the potency of honey as antibacterial agent against multiple antibiotic resistant pathogenic bacteria (Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa) with concentration of 0.2 g mL −1 . Results found that well diffusion method tends to give higher inhibitory zone than disc diffusion method but there was no correlation among the bacteria was observed except for S. Typhimurium, E. coli (R = 0.310, 0.505 and 0.316, respectively). Nan photometer assay and microtiter plates assay showed comparable results with moderately strong correlation (R 2 = 0.681 and 0.767, respectively) for S. aureus and S. typhimurium, but poor correlation was found for E. coli, B. subtilis and P. aeruginosa (R 2 = 0.441, 0.308 and 0.383, respectively). Determining the number of survivors by plating on agar after nanophotometer assay or microtiter plate assay had confirmed the effectiveness of honey as antimicrobial agent against target bacteria; which confirmed that honey has the potency to inhibit pathogens even at low concentration.American Journal of Applied Sciences 10/2014; 11(10):1773-1783.