PCR-generated artefact from 16S rRNA gene-specific primers.

Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia.
FEMS Microbiology Letters (Impact Factor: 2.05). 08/2005; 248(2):183-7. DOI:10.1016/j.femsle.2005.05.043
Source: PubMed

ABSTRACT Artefacts consisting of concatenated oligonucleotide primer sequences were generated during sub-optimally performing polymerase chain reaction amplification of bacterial 16S rRNA genes using a commonly employed primer pair. These artefacts were observed during amplification for terminal restriction fragment length polymorphism analyses of complex microbial communities, and after amplification from DNA from a microbial culture. Similar repetitive motifs were found in gene sequences deposited in GenBank. The artefact can be avoided by using different primers for the amplification reaction.

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Sep 8, 2012