Experimental Actinobacillus pleuropneumoniae infection in piglets with different types and levels of specific protection: Immunophenotypic analysis of lymphocyte subsets in the circulation and respiratory mucosal lymphoid tissue
ABSTRACT Actinobacillus pleuropneumoniae (APP) infection in piglets results in severe and fatal fibrinous hemorrhagic necrotizing pneumoniae. The aim of our study was to analyze changes in lymphocyte subset distribution in peripheral blood, bronchoalveolar lavage fluid (BALF) and tracheobronchal lymph nodes (TLN) in non-immune piglets upon a challenge with a high dose of APP and to compare the quality of such changes in unprotected piglets with counterparts exhibiting specific immunity mediated by high titers of colostrum-derived APP-specific antibodies and/or a low dose APP infection in the early postnatal period. Challenge with APP resulted in a massive increase in CD8-negative gammadelta T-cells in parallel with a reduction in numbers of CD3-CD8low cells in BALF independent of the type and level of immunity and this seems to be a general phenomenon associated with experimental infection. An increase in B-lymphocyte numbers in TLN was another characteristic feature accompanying APP infection in all experimental groups. In piglets with colostrum-derived APP-specific antibodies, this was associated with higher relative numbers of IgM+CD2+ lymphocytes in TLN, while B-cells with the CD2- surface phenotype apparently expanded in the absence of passive humoral immunity.
SourceAvailable from: Monika Vicenova[Show abstract] [Hide abstract]
ABSTRACT: Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of γδ T lymphocytes was found in pyometra samples mainly due to recruitment of γδ(+)/CD8(-) T lymphocytes. The relative expression of genes encoding selected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1β, TNF-α, IL-8, IL-17 and IFN-γ) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively.The Veterinary Journal 07/2012; 194(3). DOI:10.1016/j.tvjl.2012.05.024 · 2.17 Impact Factor
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ABSTRACT: ABSTRACTA protein chip based on surface plasmon resonance (SPR) was developed for measuring the Actinobacillus pleuropneumoniae (APP) antibody (Ab) titers using recombinant transferrin-binding protein B (Tbp B) as an antigen. The diagnostic potential of this SPR assay for detecting the Ab titers to APP Tbp B was compared with that of enzyme-linked immunosorbent assay (ELISA) using 70 serum samples obtained from six pig farms. The SPR assay was found to be highly specific and sensitive. Moreover, there was a strong positive correlation between the SPR and ELISA titers (n = 70, r = 0.858, P < 0.01). In conclusion, the SPR, which is a label-free method, is expected to be a valuable and reproducible tool in the serodiagnosis of an APP infection.Journal of Rapid Methods & Automation in Microbiology 05/2007; 15(2):206 - 215. DOI:10.1111/j.1745-4581.2007.00065.x · 0.59 Impact Factor
Bulletin- Veterinary Institute in Pulawy 01/2006; 50:263-267. · 0.37 Impact Factor