A rapid and potent DNA vaccination strategy defined by in vivo monitoring of antigen expression

Johns Hopkins University, Baltimore, Maryland, United States
Nature Medicine (Impact Factor: 28.05). 09/2005; 11(8):899-904. DOI: 10.1038/nm1264
Source: PubMed

ABSTRACT Induction of immunity after DNA vaccination is generally considered a slow process. Here we show that DNA delivery to the skin results in a highly transient pulse of antigen expression. Based on this information, we developed a new rapid and potent intradermal DNA vaccination method. By short-interval intradermal DNA delivery, robust T-cell responses, of a magnitude sufficient to reject established subcutaneous tumors, are generated within 12 d. Moreover, this vaccination strategy confers protecting humoral immunity against influenza A infection within 2 weeks after the start of vaccination. The strength and speed of this newly developed strategy will be beneficial in situations in which immunity is required in the shortest possible time.

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Available from: Monika C Wolkers, Jul 02, 2015
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