Article

Real-time polymerase chain reaction of immunoglobulin rearrangements for quantitative evaluation of minimal residual disease in myeloma.

Divisione di Ematologia, Universita' di Torino, Torno, Italy.
Methods in molecular medicine 02/2005; 113:145-63. DOI:10.1385/1-59259-916-8:145 pp.145-63
Source: PubMed

ABSTRACT The evaluation of minimal residual disease (MRD) is critical in the evaluation of treatments aimed at maximal cytoreduction in multiple myeloma (MM). Qualitative evaluation of MRD now has a 10-yr-long history, but it remains a relatively sophisticated procedure. More recently, real-time quantitative approaches have also been developed. These approaches allow a very effective monitoring of disease but introduce additional complexity and costs to the procedure. This chapter describes how we currently perform real-time polymerase chain reaction (PCR) in MM. Compared to the first description of the assay in June 2000, significant improvements have been made. Although real-time PCR is the main focus of the chapter, most of the information suitable for a proper setup of a qualitative approach is also provided.

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Keywords

10-yr-long history
 
additional complexity
 
assay
 
effective monitoring
 
first description
 
maximal cytoreduction
 
minimal residual disease
 
qualitative approach
 
Qualitative evaluation
 
real-time polymerase chain reaction
 
significant improvements
 
sophisticated procedure