Effect of Different Carbon Sources on Endochitinase Production by Colletotrichum gloeosporioides

Departamento de Microbiologia Geral, Instituto de Microbiologia Professor Paulo de Góes (IMPPG), CCS, Bloco I,Universidade Federal do Rio Janeiro (UFRJ), Ilha do Fundão, 21941-590, Rio de Janeiro, RJ, Brazil.
Current Microbiology (Impact Factor: 1.42). 08/2005; 51(1):16-21. DOI: 10.1007/s00284-005-4506-9
Source: PubMed


The present work analyzes the production of endochitinase by Colletotrichum gloeosporioides, a phytopathogenic fungus, using six different carbon sources and two pH values. For quantitative assay of endochitinase activity in solution, the synthetic substrate 4-methylumbelliferyl-beta-D-N,N',N"-triacetylchitotrioside was used. The major productions were obtained at pH 7.0 and 9.0, when colloidal chitin and glucose were used, whereas xylose and lactose were not good carbon sources. When testing different concentrations of colloidal chitin, glucose and glucosamine, colloidal chitin 0.5% was the best substrate, giving values of 2.4 U at the fifth day. When using glucose, best production occurred at 0.3% concentration, after 5 days growth, with values of 1.31 U. Endochitinase production was markedly decreased in high levels of glucose and in all glucosamine concentrations tested. SDS-PAGE co-polymerized with glycol-chitin analysis showed three major activity bands of 200, 100, and 95 kDa, when incubated at 50 degrees C.

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    • "Microbes are potential producers of chitinolytic enzymes and medium composition can significantly affect their production [26]. The use of statistical methods based on experimental design, such as the RSM, enabled the evaluation of the simultaneous effects of culture medium components and of variables (e.g. "
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    Journal of Basic Microbiology 05/2013; 53(5). DOI:10.1002/jobm.201100590 · 1.82 Impact Factor
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    • "Several microorganisms are potential producers of chitinolytic and proteolytic enzymes, and medium composition can significantly affect their production (Nawani and Kapadnis 2005; Souza et al. 2005; Lopes et al. 2008). "
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    Annals of Microbiology 09/2011; 62(3). DOI:10.1007/s13213-011-0371-x · 0.99 Impact Factor
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    • "Among three media tested, colloidal chitin amended YNB supported high chitinase production than LBB and NB. Concentration of colloidal chitin is another vital factor as it is reported to induce the chitinase production in several microorganisms (Frandberg and Schnurer, 1994; Mathivanan et al., 1997, 1998; Soiuza et al., 2005). At 0.3% concentrations, colloidal chitin significantly enhanced the chitinase activity. "
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