Genome Scale Identification of Treponema pallidum Antigens

Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
Infection and Immunity (Impact Factor: 3.73). 08/2005; 73(7):4445-50. DOI: 10.1128/IAI.73.7.4445-4450.2005
Source: PubMed


Antibody responses for 882 of the 1,039 proteins in the proteome of Treponema pallidum were examined. Sera collected from infected rabbits were used to systematically identify 106 antigenic proteins, including
22 previously identified antigens and 84 novel antigens. Additionally, sera collected from rabbits throughout the course of
infection demonstrated a progression in the breadth and intensity of humoral immunoreactivity against a representative panel
of T. pallidum antigens.

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    • "Similar variability in the number of invariant repetitions was found in the TP0470 gene (Mikalová et al., 2010; Strouhal et al., 2007). Moreover, the conserved hypothetical protein TP0470 was found to be immunogenic (Brinkman et al., 2006; McKevitt et al., 2005). "
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    ABSTRACT: Pathogenic uncultivable treponemes, similar to syphilis-causing Treponema pallidum subspecies pallidum, include T. pallidum ssp. pertenue, T. pallidum ssp. endemicum and Treponema carateum, which cause yaws, bejel and pinta, respectively. Genetic analyses of these pathogens revealed striking similarity among these bacteria and also a high degree of similarity to the rabbit pathogen, Treponema paraluiscuniculi, a treponeme not infectious to humans. Genome comparisons between pallidum and non-pallidum treponemes revealed genes with potential involvement in human infectivity, whereas comparisons between pallidum and pertenue treponemes identified genes possibly involved in the high invasivity of syphilis treponemes. Genetic variability within syphilis strains is considered as the basis of syphilis molecular epidemiology with potential to detect more virulent strains, whereas genetic variability within a single strain is related to its ability to elude the immune system of the host. Genome analyses also shed light on treponemal evolution and on chromosomal targets for molecular diagnostics of treponemal infections.
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    • "Among them, BP2818 and putative exported solute binding protein were included in the OMV proteome of B. p, and only Sbp was identified as an immunogenic protein in both TMP and ECP fractions, indicating its highly reliable immunogenicity. The D-isomer specific 2-hydroxyacid dehydrogenase exhibits more than 50% sequence similarity to putative 2-hydroxyacid dehydrogenase which was identified as one of the antigenic proteins binding to serum antibody obtained from treponema pallidum-infected rabbits [82]. Pfam analysis revealed that BP2818 and BP3575 belong to the Lipoprotein_9 and ANF_receptor families, respectively. "
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    • "With protein arrays, the limitation is based on the yield of the lowest expressed protein and quantities are always finite. Nonetheless , partial protein arrays have been developed for a few bacteria including Yersinia pestis [26], Treponema pallidum [27], and Chlamydia trachomatis [28]. Once constructed, protein arrays are very powerful tools that enable the direct comparison of unknown proteins with known antigens across an even platform. "
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