Article

Preparative isolation and purification of rupestonic acid from the Chinese medicinal plant Artemisia rupestris L. by high-speed counter-current chromatography.

Xinjiang Technical Institute of Physics & Chemistry, CAS, Urumqi 830011, China.
Journal of Chromatography (impact factor: 4.53). 06/2005; 1076(1-2):198-201. pp.198-201
Source: PubMed

ABSTRACT Rupestonic acid was purified for the first time by high-speed counter-current chromatography from a dichloromethane extract of the traditional Chinese medicinal plant Artemisia rupestris L. The separation was performed in two steps with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (6:4:3.5:6.5, v/v) with 0.5% acetic acid in stationary-phase. From 200 mg of the crude extract, 27.9 mg of rupestonic acid was obtained at over 98% purity as determined by HPLC analysis, and its chemical structure was confirmed by MS, 1H and 13C nuclear magnetic resonance.

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Keywords

13C nuclear magnetic resonance
 
high-speed counter-current chromatography
 
stationary-phase
 
steps
 
traditional Chinese medicinal plant Artemisia rupestris L