Hand is a direct target of Tinman and GATA factors during Drosophila cardiogenesis and hematopoiesis
ABSTRACT The existence of hemangioblasts, which serve as common progenitors for hematopoietic cells and cardioblasts, has suggested a molecular link between cardiogenesis and hematopoiesis in Drosophila. However, the molecular mediators that might link hematopoiesis and cardiogenesis remain unknown. Here, we show that the highly conserved basic helix-loop-helix (bHLH) transcription factor Hand is expressed in cardioblasts, pericardial nephrocytes and hematopoietic progenitors. The homeodomain protein Tinman and the GATA factors Pannier and Serpent directly activate Hand in these cell types through a minimal enhancer, which is necessary and sufficient to drive Hand expression in these different cell types. Hand is activated by Tinman and Pannier in cardioblasts and pericardial nephrocytes, and by Serpent in hematopoietic progenitors in the lymph gland. These findings place Hand at a nexus of the transcriptional networks that govern cardiogenesis and hematopoiesis, and indicate that the transcriptional pathways involved in development of the cardiovascular, excretory and hematopoietic systems may be more closely related than previously appreciated.
- SourceAvailable from: Caroline Kumsta
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- "Thus, severely compromised integrin/ ILK pathway function is detrimental for the heart, but fine-tuned moderate reduction maintains youthful cardiac performance, suggesting a dual role for this complex in regulating cardiac integrity and aging. Results ilk heterozygous mutants have extended lifespan in Drosophila As the RNAi-mediated KD of ilk extends lifespan in C. elegans (Hansen et al., 2005; Curran & Ruvkun, 2007; Kumsta et al., 2014), we wondered whether reduced ilk expression is also beneficial to longevity in Drosophila. As lifespan can be significantly modulated by genetic background (Grandison et al., 2009), we first backcrossed ilk 54 mutants (premature stop codon; Zervas et al., 2011 "
ABSTRACT: Cardiac performance decreases with age, which is a major risk factor for cardiovascular disease and mortality in the aging human population, but the molecular mechanisms underlying cardiac aging are still poorly understood. Investigating the role of integrin-linked kinase (ilk) and β1-integrin (myospheroid, mys) in Drosophila, which colocalize near cardiomyocyte contacts and Z-bands, we find that reduced ilk or mys function prevents the typical changes of cardiac aging seen in wildtype, such as arrhythmias. In particular, the characteristic increase in cardiac arrhythmias with age is prevented in ilk and mys heterozygous flies with nearly identical genetic background, and they live longer, in line with previous findings in Caenorhabditis elegans for ilk and in Drosophila for mys. Consistent with these findings, we observed elevated β1-integrin protein levels in old compared with young wild-type flies, and cardiac-specific overexpression of mys in young flies causes aging-like heart dysfunction. Moreover, moderate cardiac-specific knockdown of integrin-linked kinase (ILK)/integrin pathway-associated genes also prevented the decline in cardiac performance with age. In contrast, strong cardiac knockdown of ilk or ILK-associated genes can severely compromise cardiac integrity, including cardiomyocyte adhesion and overall heart function. These data suggest that ilk/mys function is necessary for establishing and maintaining normal heart structure and function, and appropriate fine-tuning of this pathway can retard the age-dependent decline in cardiac performance and extend lifespan. Thus, ILK/integrin-associated signaling emerges as an important and conserved genetic mechanism in longevity, and as a new means to improve age-dependent cardiac performance, in addition to its vital role in maintaining cardiac integrity.Aging cell 01/2014; 13(3). DOI:10.1111/acel.12193 · 5.94 Impact Factor
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- "Similar Figure 2. jumu and CHES-1-like Embryonic Expression and Loss-of-Function Cardiac Phenotypes (A and B) jumu (A) and CHES-1-like (B) mRNAs are expressed in the cardiac mesoderm at embryonic stage 11 (arrows). (C–E) Whole embryo RNAi results for dsRNA corresponding to lacZ (C), jumu (D), and CHES-1-like (E) in live embryos in which CCs express a nuclear localized form of GFP under control of a Hand enhancer (Han and Olson, 2005), and PCs express both Hand-GFP nuc and a nuclear form of DsRed under control of a heart enhancer from the Him gene (Him-DsRED nuc ; A.M.M. and S. Michaud, unpublished data). Arrows indicate incorrect numbers and uneven distribution of CCs and PCs. "
ABSTRACT: The development of a complex organ requires the specification of appropriate numbers of each of its constituent cell types, as well as their proper differentiation and correct positioning relative to each other. During Drosophila cardiogenesis, all three of these processes are controlled by jumeau (jumu) and Checkpoint suppressor homologue (CHES-1-like), two genes encoding forkhead transcription factors that we discovered utilizing an integrated genetic, genomic, and computational strategy for identifying genes expressed in the developing Drosophila heart. Both jumu and CHES-1-like are required during asymmetric cell division for the derivation of two distinct cardiac cell types from their mutual precursor and in symmetric cell divisions that produce yet a third type of heart cell. jumu and CHES-1-like control the division of cardiac progenitors by regulating the activity of Polo, a kinase involved in multiple steps of mitosis. This pathway demonstrates how transcription factors integrate diverse developmental processes during organogenesis.Developmental Cell 07/2012; 23(1):97-111. DOI:10.1016/j.devcel.2012.05.011 · 10.37 Impact Factor
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- "12 and UAS-jumu (Strodicke et al., 2000; Hofmann et al., 2009); TinD-GAL4 (Yin et al., 1997); twi-GAL4 (Greig and Akam, 1993); and Hand-GAL4 (Han and Olson, 2005). Mutant chromosomes were maintained over the TM3, ftz-lacZ balancer. "
ABSTRACT: A common theme in developmental biology is the repeated use of the same gene in diverse spatial and temporal domains, a process that generally involves transcriptional regulation mediated by multiple separate enhancers, each with its own arrangement of transcription factor (TF)-binding sites and associated activities. Here, by contrast, we show that the expression of the Drosophila Nidogen (Ndg) gene at different embryonic stages and in four mesodermal cell types is governed by the binding of multiple cell-specific Forkhead (Fkh) TFs - including Biniou (Bin), Checkpoint suppressor homologue (CHES-1-like) and Jumeau (Jumu) - to three functionally distinguishable Fkh-binding sites in the same enhancer. Whereas Bin activates the Ndg enhancer in the late visceral musculature, CHES-1-like cooperates with Jumu to repress this enhancer in the heart. CHES-1-like also represses the Ndg enhancer in a subset of somatic myoblasts prior to their fusion to form multinucleated myotubes. Moreover, different combinations of Fkh sites, corresponding to two different sequence specificities, mediate the particular functions of each TF. A genome-wide scan for the occurrence of both classes of Fkh domain recognition sites in association with binding sites for known cardiac TFs showed an enrichment of combinations containing the two Fkh motifs in putative enhancers found within the noncoding regions of genes having heart expression. Collectively, our results establish that different cell-specific members of a TF family regulate the activity of a single enhancer in distinct spatiotemporal domains, and demonstrate how individual binding motifs for a TF class can differentially influence gene expression.Development 02/2012; 139(8):1457-66. DOI:10.1242/dev.069005 · 6.27 Impact Factor