In vivo imaging of amyloid-beta deposits in mouse brain with multiphoton microscopy. Methods Mol Biol

Department of Neurology, Massachusetts General Hospital, Charlestown, MA, USA.
Methods in Molecular Biology (Impact Factor: 1.29). 02/2005; 299:349-63.
Source: PubMed


With the advent of transgenic mouse models expressing cortical amyloid pathology, the potential to study its progression in an intact brain has been realized. Multiphoton microscopy provides a non-destructive means of imaging with micron resolution up to 500 microm deep into the cortex. We detail a surgical procedure and discuss a multiphoton imaging approach that allows for labeling and chronic visualization of amyloid-beta deposits through a cranial window. The ability to monitor these hallmarks of Alzheimer's disease enables studies aimed at evaluating the efficacy of treatment and prevention strategies.

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    • "MPFM has been combined with in vivo fluorescence probes for cellualr imaging in diverse organs such as skin [141], kidney [142], heart [143], and brain [144]. It has also been applied as a tool to study the development, progression and potential treatment of pathological conditions such as cancer [145] and Alzheimer's disease [146]. In tumor biology for instance, MPFM of transgenic mice with GFP-fluorescent cells has been used to track single cell behavior within different tumor microenvironments [139] [147]. "
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    • "The uniquely designed PCA presented here has the advantage to detect and visualize α-syn oligomerization in post mortem tissue with minimal tissue processing, but could potentially be utilized to monitor the formation of α-syn oligomers over time in the brain of a living animal using two-photon microscopy. Recent advances in two-photon microscopy have enabled in vivo visualization of protein aggregation and neurodegeneration in the brain of an Alzheimer’s disease mouse model [22,33,34] and protein degradation in the brain of a PD animal model [35-37]. Here, using two-photon microscopy, we demonstrate the ability to image and detect α-syn oligomers in vivo. "
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    05/2013; 1(1):6. DOI:10.1186/2051-5960-1-6
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    • "The general techniques for cranial window preparation and in vivo multiphoton imaging have been published previously (Skoch et al., 2005; Holtmaat et al., 2009). In this study, we have used a modified approach, developed by our group, to optimize the application of blood-brain-barrier impermeant agents to cortical areas (Unni et al.). "
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