Impact of Common Polymorphisms in Candidate Genes for Insulin Resistance and Obesity on Weight Loss of Morbidly Obese Subjects after Laparoscopic Adjustable Gastric Banding and Hypocaloric Diet

Dipartimento Medicina Sperimentale e Clinica, Università Magna-Graecia di Catanzaro, Via Campanella, 115, 88100 Catanzaro, Italy.
Journal of Clinical Endocrinology &amp Metabolism (Impact Factor: 6.21). 10/2005; 90(9):5064-9. DOI: 10.1210/jc.2005-0404
Source: PubMed


It is unknown whether genetic factors that play an important role in body weight homeostasis influence the response to laparoscopic adjustable gastric banding (LAGB).
We investigated the impact of common polymorphisms in four candidate genes for insulin resistance on weight loss after LAGB.
The design was a 6-month follow-up study. Setting: The study setting was hospitalized care.
A total of 167 unrelated morbidly obese subjects were recruited according to the following criteria: age, 18-66 yr inclusive; and body mass index greater than 40 kg/m2 or greater than 35.0 kg/m2 in the presence of comorbidities. Intervention: LAGB was used as an intervention.
Measure of correlation between weight loss and common polymorphisms in candidate genes for insulin resistance and obesity was the main outcome measure.
The following single nucleotide polymorphisms were detected by digestion of PCR products with appropriate restriction enzymes: Gly972Arg of the insulin receptor substrate-1 gene, Pro12Ala of the proliferator-activated receptor-gamma gene, C-174G in the promoter of IL-6 gene, and G-866A in the promoter of uncoupling protein 2 gene. Baseline characteristics including body mass index did not differ between the genotypes. At the 6-month follow-up after LAGB, carriers of G-174G IL-6 genotype had lost more weight than G-174C or C-174C genotype (P = 0.037), and carriers of A-866A uncoupling protein 2 genotype had lost more weight as compared with G-866G (P = 0.018) and G-866A (P = 0.035) genotype, respectively. Weight loss was lower in carriers of Gly972Arg insulin receptor substrate-1 genotype than Gly972Gly carriers, but not statistically significant (P = 0.06). No difference between carriers of Pro12Ala and Pro12Pro proliferator-activated receptor-gamma genotype was observed.
These data demonstrate that genetic factors, which play an important role in the regulation of body weight, may account for differences in the therapeutic response to LAGB.

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    • "itro ( Esterbauer et al . , 2001 ) and , could therefore , have a functional like effect by decreasing UCP2 transcription . However , this functionality effect was not observed in vivo ( Walder et al . , 1998 ) . Previous studies have , although with discrepancy , found an association between weight loss and other poly morphisms in the UCP2 gene ( Sesti et al . , 2005 ; Cha et al . , 2007 ; Chen et al . , 2007 ) . The possibility cannot be excluded that these polymorphisms may be in linkage disequilibrium with UCP2 D / I , or that all these associations may be the result of a yet to be discovered functional polymorphism in the UCP2 gene or in nearby genes ( Yoon et al . , 2007 ; De Luis et al . , 200"
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    • "Patients homozygous for the C allele had increases in IL-6 levels post-surgery, whereas G carriers had decreases. In an alternate study of weight loss following gastric banding surgery, homozygous carriers of the G allele lost significantly more weight than GC or CC subjects (Sesti et al., 2005). This suggests that GG carriers may respond more favorably to gastric surgery interventions (that is, better decreases in both body weight and inflammation). "
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    • "As mentioned earlier, some studies have found a participation of the -174G . C polymorphism on weight loss (Poitou et al. 2005; Sesti et al. 2005), although the association of this variant with long-term weight maintenance has apparently not been studied yet. The present study evidenced a protective effect of the IL-6 C allele against weight regain after a weight loss induced by a LED. "
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