Article

Culture supernatant of Lactobacillus acidophilus stimulates proliferation of embryonic cells.

Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA.
Experimental Biology and Medicine (impact factor: 2.64). 08/2005; 230(7):494-500. pp.494-500
Source: PubMed

ABSTRACT Our previous report showed that supernatants of Lactobacillus acidophilus (LS) cultures possessed chemotactic and angiogenic properties. Specifically, LS stimulated gene expression and the secretion of tumor necrosis factor-alpha (TNF-alpha), the proliferation of immune cells in vitro, and blood vessel formation. Chemotaxis and proliferation of inflammatory cells in vivo were also stimulated by LS. In the current study, we hypothesized that LS stimulates the growth and development of other rapidly dividing cells, including embryonic cells. The stimulatory effects of LS on a neuroblastoma cell line (Neuro-2a), chicken embryos, and bovine embryos were examined. The addition of LS to Neuro-2a cultures caused a proliferation of cells in a concentration-dependent manner. Pretreatment of LS at 56 degrees C for 30 mins did not affect its stimulatory activity. The administration of LS to the chorioallantoic membrane (CAM) of chicken-embryonated eggs for 1-2 days resulted in extensive thickening of the membrane. The thickening was due to the influx and proliferation of fibroblasts and inflammatory cells, the accumulation of loose connective tissue composed primarily of mucopolysaccharides, and/or the formation of blood vessels. Stimulatory effects of LS on bovine embryos were also observed. The treatment with LS significantly promoted the development of zygotes to the four-cell stage and from the four-cell stage to blastocysts. These results have confirmed our hypothesis that LS exerts a stimulatory effect on the cells of embryonic stages including neuroblastoma cells, the CAM of chicken embryos, and bovine embryos from zygotes to blastocysts.

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Keywords

56 degrees C
 
angiogenic properties
 
blood vessel formation
 
chicken-embryonated eggs
 
concentration-dependent manner
 
dividing cells
 
embryonic cells
 
extensive thickening
 
four-cell stage
 
gene expression
 
immune cells
 
inflammatory cells
 
Lactobacillus acidophilus
 
loose connective tissue
 
LS exerts
 
LS stimulates
 
neuroblastoma cell line
 
neuroblastoma cells
 
Stimulatory effects
 
tumor necrosis factor-alpha