Article

p66(shc) is highly expressed in fibroblasts from centenarians.

Department of Experimental Pathology, University of Bologna, via S. Giacomo 12, 40126 Bologna, Italy.
Mechanisms of Ageing and Development (impact factor: 3.44). 09/2005; 126(8):839-44. DOI:10.1016/j.mad.2005.03.004 pp.839-44
Source: PubMed

ABSTRACT p66(shc-/-) mice exhibit prolonged lifespan and increased resistance to oxidative and hypoxic stress. To investigate p66(shc) involvement in human longevity, p66(shc) mRNA and protein were evaluated in fibroblasts from young people, elderly and centenarians, exposed to oxidative or hypoxic stress. Unexpectedly, centenarians showed the highest basal levels of p66(shc). Oxidative stress induced p66(shc) in all samples. At variance, hypoxic stress caused p66(shc) reduction only in cells from centenarians. These changes occurred in absence of any modification of p66(shc) promoter methylation pattern. Intriguingly, in cells from centenarians, p66(shc) induction was affected by p53 codon 72 polymorphism. Thus, cells from centenarians present a peculiar regulation of p66(shc), suggesting that its role in mammalian longevity is more complex than previously thought.

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Keywords

centenarians
 
centenarians present
 
highest basal levels
 
hypoxic stress
 
Intriguingly
 
mammalian longevity
 
oxidative
 
Oxidative stress induced p66(shc)
 
p53 codon 72 polymorphism
 
peculiar regulation
 
variance
 
young people