The Dynein Light Chain Tctex-1 Has a Dynein-Independent Role in Actin Remodeling during Neurite Outgrowth

Department of Ophthalmology, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10012, USA.
Developmental Cell (Impact Factor: 10.37). 08/2005; 9(1):75-86. DOI: 10.1016/j.devcel.2005.04.003
Source: PubMed

ABSTRACT Coordinated microtubule and microfilament changes are essential for the morphological development of neurons; however, little is know about the underlying molecular machinery linking these two cytoskeletal systems. Similarly, the indispensable role of RhoGTPase family proteins has been demonstrated, but it is unknown how their activities are specifically regulated in different neurites. In this paper, we show that the cytoplasmic dynein light chain Tctex-1 plays a key role in multiple steps of hippocampal neuron development, including initial neurite sprouting, axon specification, and later dendritic elaboration. The neuritogenic effects elicited by Tctex-1 are independent from its cargo adaptor role for dynein motor transport. Finally, our data suggest that the selective high level of Tctex-1 at the growth cone of growing axons drives fast neurite extension by modulating actin dynamics and also Rac1 activity.

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Available from: Cecilia Conde, Jul 01, 2015
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