Bioactive pectic polysaccharides from Glinus oppositifolius (L.) Aug. DC., a Malian medicinal plant, isolation and partial characterization
ABSTRACT Glinus oppositifolius (L.) Aug. DC. (Aizoaceae) is a Malian medicinal plant used against various types of illnesses related to the immune response, like joint pains, inflammations, fever, malaria and wounds. Two pectin type polysaccharides, GOA1 and GOA2, being isolated from a 50 degrees C water extract from the aerial parts of Glinus oppositifolius were investigated for their activity towards the complement system and different leukocyte subsets because of the assumed effects on conditions related to the immune system. The polysaccharide polymer in GOA1 was shown to contain considerable amounts of the neutral sugars arabinose (26.4 mol%) and galactose (42.9 mol%), and methylation analysis indicated the presence of arabinogalactans type I (AG-I) and type II (AG-II). GOA2 was rich in galacturonic acid (68.3 mol%), along with rhamnose, arabinose and galactose. Structural studies indicated that rhamnose and galacturonic acid might constitute a rhamnogalacturonan backbone, often found in pectic substances, with side chains consisting of arabinose and galactose. Both GOA1 and GOA2 were shown to exhibit potent dose-dependent complement fixating activities, and induced chemotaxis of macrophages, T cells and NK cells.
Full-textDOI: · Available from: Terje E Michaelsen, Aug 02, 2015
- SourceAvailable from: Martin Polovka
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- "Fraction EP2/C comprised polygalacturonan chains with a very low proportion of ramified RG I regions, whereas, the most active DP4/C was built of partially methylesterified homogalacturonan chains with branched RG I regions (Košt'álová et al., 2011). The results were in accord with previous studies on immunoactive pectins isolated from various herbal plants (Yamada and Kiyohara, 1999; Paulsen and Barsett, 2005; Glaeserud et al., 2011; Inngjerdingen et al., 2005 "
ABSTRACT: Pectic polysaccharides have attracted great attention due to their health-promoting potential. From the Styrian oil-pumpkin biomass representing an alternative pectin source, two series of acidic polysaccharide fractions were isolated using in succession hot water, EDTA and dilute HCl. Chemical and spectroscopic (FTIR, NMR) analyses of the fractions revealed the predominance of partially methylesterified and acetylated pectins containing homogalacturonan and ramified rhamnogalacturonan elements, and a minority of phenolic compounds, protein and hemicelluloses. The pectins exhibited moderate antioxidant activities tested by colorimetric DPPH and FRAP assays and EPR method. The activities correlated with the total phenolic content. In the complement-fixing test, most of the pectin fractions exhibited potent effects comparable to the positive control – a pectin from Plantago major. The oil-pumpkin pectins represent potential antioxidant and immunoenhancing additives applicable in food and nutraceuticals.Industrial Crops and Products 01/2013; 41(1):127–133. DOI:10.1016/j.indcrop.2012.04.029 · 2.84 Impact Factor
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- "Enhydra fluctuans was reported for antioxidant, analgesic, antimicrobial activity, and antibacterial activity     . Glinus oppositifolius (L.) was found to contain immunomodulating peptic polymer, terpinoid saponine having antiprotozoal activity, and bioactives having antioxidant property    . "
ABSTRACT: To investigate the antibacterial and cytotoxic activity of fourteen different edible vegetables methanolic extract from Bangladesh. The antibacterial activity was evaluated using disc diffusion assay method against 12 bacteria (both gram positive and gram negative). The plant extracts were also screened for cytotoxic activity using the brine shrimp lethality bioassay method and the lethal concentrations (LC50) were determined at 95% confidence intervals by analyzing the data on a computer loaded with "Finney Programme". All the vegetable extracts showed low to elevated levels of antibacterial activity against most of the tested strains (zone of inhibition=5-28 mm). The most active extract against all bacterial strains was from Xanthium indicum which showed remarkable antibacterial activity having the diameter of growth inhibition zone ranging from 12 to 28 mm followed by Alternanthera sessilis (zone of inhibition=6-21 mm). All extracts exhibited considerable general toxicity towards brine shrimps. The LC50 value of the tested extracts was within the range of 8.447 to 60.323 µg/mL with respect to the positive control (vincristine sulphate) which was 0.91 µg/mL. Among all studied extracts, Xanthium indicum displayed the highest cytotoxic effect with LC50 value of 8.447 µg/mL. The results of the present investigation suggest that most of the studied plants are potentially good source of antibacterial and anticancer agents.Asian Pacific Journal of Tropical Biomedicine 01/2013; 3(1):1-7. DOI:10.1016/S2221-1691(13)60015-5
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- "e l s e v i e r . c o m / l o c a t e / f o o d c h e m compounds from medicinal plants used by traditional healers in Mali has shown that type II AGs are present in a number of plants used for wound-healing (Diallo, Paulsen, Liljeback, & Michaelsen, 2003; Inngjerdingen et al., 2005; Togola et al., 2008). However, interpretation of the structure–activity relationships in these studies is limited, because the extracts are complex mixtures of polysaccharides that contain varying amounts of AGPs, as judged by interaction with b-glucosyl Yariv reagent (Van Holst & Clarke, 1985). "
ABSTRACT: Fractionation of manuka, kanuka and clover honeys indicated the >10kDa fraction contained small amounts of type II arabinogalactans (AGs), which are often present as arabinogalactan proteins (AGPs). AGPs were isolated from the >10kDa fraction of kanuka honey using β-glucosyl Yariv reagent and their composition and structure analysed. Constituent sugar, glycosyl linkage and NMR spectroscopy analysis of the purified AGP fraction revealed a predominance of neutral sugars, mainly galactose and arabinose, linked in a highly-branched structure typical of type II AGs. The molecular weight of the major component of the purified AGPs was ∼110kDa, as determined by size-exclusion chromatography-multi-angle laser light scattering (SEC-MALLS). The Yariv supernatant fraction contained less total sugar, especially galactose, and more protein than purified AGPs. Linkage analysis indicated this fraction also contained an AG-type polymer in addition to various other polysaccharides and SEC-MALLS indicated the molecular weight of the major component was ∼32kDa.Food Chemistry 10/2011; 128(4):949-956. DOI:10.1016/j.foodchem.2011.03.124 · 3.26 Impact Factor