Effects of sanguinaria in fluoride-containing dentifrices on the remineralisation of subsurface carious lesion in vitro.
ABSTRACT The purpose of this study was to investigate the potential cariostatic benefits of dentifrice containing both fluoride (F) and sanguinaria (SG) in a silica abrasive formula using the microbial de- and re-mineralisation cycle model.
Samples of bovine tooth enamel were embedded in resin, polished, exposed to a lactate carbopol buffer system for 44 hours, and divided into one control and five test groups of 12 specimens each by randomised blocks with VHN stratification. Surface microhardness was compared before and after the microbial cyclic sequences.
VHNs for groups treated with dentifrices containing 1,000 ppm F from MFP with or without bamboo salt (BS) were significantly higher than the control. The groups treated with dentifrices containing F from NaF/silica with or without SG had significantly higher VHNs than that with F from MFP/DCPD. The VHNs treated with dentifrice containing both NaF-SG demonstrated the highest among test groups, indicating that there may be a synergistic effect in remineralising the incipient subsurface lesion.
The dentifrice containing both NaF and SG showed a significantly greater effect (p < 0.05) on the remineralisation of the enamel lesion than the other test dentifrices.
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ABSTRACT: The inhibitory effect of fluorapatite (FAP)-derived fluoride upon resting cell suspensions of Streptococcus mutans incubated at pH 4.5 and 6.5 was studied using lactic acid production from 0.1% sucrose as an indicator of fermentation activity. Cells incubated with FAP produced significantly less lactic acid than did cells incubated with hydroxyapatite (HAP). Addition of HAP to cell suspensions containing FAP reduced this inhibition, suggesting that dissolution of the FAP was necessary for inhibition. Incubation with low concentrations of NaF showed significant inhibition in cell suspensions incubated with as little as 0.45 micrograms/mL F at pH 5.0. These results provide further support to the hypothesis that fluoride levels in plaque and enamel, achievable through use of fluoridated water and/or fluoride dentifrices, may produce appreciable inhibition of glycolysis at the acidic pH levels which are readily achieved in plaque. Thus, bacterial acid production may activate plaque and enamel-bound fluoride, resulting in inhibition of further acid production, and thereby contribute substantially to the other cariostatic mechanisms of fluoride.Journal of Dental Research 02/1986; 65(1):30-3. · 3.83 Impact Factor
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ABSTRACT: In this study, the differences in lesion remineralization and fluoride uptake after brushing with a 300 ppm F or a 1000 ppm F dentifrice (as NaF) were investigated. Twenty volunteers with partial dentures in their lower jaws were divided into two groups. Bovine enamel slabs with artificial lesions were mounted in the dentures. After a test period of six weeks, the slabs were taken out and analyzed. There was no statistically significant difference between the two groups, in F-uptake and susceptibility of the enamel to demineralization, although the findings favored the 1000 ppm F toothpaste. The microradiograms in the 300 ppm F group showed lesions more pronounced than those in the 1000 ppm group. The present study cannot support the presumption that the fluoride content of regular NaF dentifrices can be lowered to 300 ppm F without undesirable effects on lesion arrest and remineralization.Journal of Dental Research 01/1987; 65(12):1410-4. · 3.83 Impact Factor
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ABSTRACT: Acid-softened bovine enamel slabs were mounted in acrylic mandibular removable appliances and worn by 8 male subjects. Control slabs were worn for 4 days without exposure to topical fluoride agents. The test slabs were brushed 4 times a day for 3 days with a 0.24% NaF dentifrice or rinsed 4 times a day for 3 days with a 0.02% APF mouthrinse and worn a 4th day without exposure to the fluoride agent. Microhardness values for both groups of test slabs were significantly higher than controls following intraoral exposure and after in vitro exposure to acid. The uptake of fluoride by both mouthrinse- and dentifrice-treated slabs was significantly higher than the controls.Copyright © 1986 S. Karger AG, BaselCaries Research 02/1986; 20(1):48-55. · 2.51 Impact Factor