Molecular imaging using labeled donor tissues reveals patterns of engraftment, rejection, and survival in transplantation

Department of Pediatrics, Stanford School of Medicine, Stanford, California, USA.
Transplantation (Impact Factor: 3.78). 08/2005; 80(1):134-9. DOI: 10.1097/01.TP.0000164347.50559.A3
Source: PubMed

ABSTRACT Tissue regeneration and transplantation of solid organs involve complex processes that can only be studied in the context of the living organism, and methods of analyzing these processes in vivo are essential for development of effective transplantation and regeneration procedures. We utilized in vivo bioluminescence imaging (BLI) to noninvasively visualize engraftment, survival, and rejection of transplanted tissues from a transgenic donor mouse that constitutively expresses luciferase. Dynamic early events of hematopoietic reconstitution were accessible and engraftment from as few as 200 transplanted whole bone marrow (BM) cells resulted in bioluminescent foci in lethally irradiated, syngeneic recipients. The transplantation of autologous pancreatic Langerhans islets and of allogeneic heart revealed the tempo of transplant degeneration or immune rejection over time. This imaging approach is sensitive and reproducible, permits study of the dynamic range of the entire process of transplantation, and will greatly enhance studies across various disciplines involving transplantation.

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    • "This early release of cytokines activates antigen presenting cells (APCs), resulting in the activation and proliferation of allo-reactive T cells (Ferrara et al., 2009). Bioluminescence imaging (BLI) is a sensitive tool that enables noninvasive in vivo monitoring of cells and provides important information on the biodistribution, proliferation, and persistence of cells (Cao et al., 2005; Panoskaltsis-Mortari et al., 2004; Weissleder and Pittet, 2008). For this reason, this technique has been used to track immune cells during GVHD, usually under MHC-mismatched conditions. "
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    • "Mice used in the study were 6-to 8-week-old wild-type Swiss CD1 males, transgenic mice expressing ubiquitously GFP [30], or GFP and firefly luciferase [31] from colonies maintained in our institutional animal facility. All experimental procedures were performed according to the guidelines of the Italian National Institutes of Health and were approved by the Institutional Animal Care and Use Committee. "
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    12/2010; 2011(1687-966X):304562. DOI:10.4061/2011/304562
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    • "In addition, while housekeeping promoters are widely used in cell culture to achieve uniform and high-level expression, they have proved problematic in transgenic animals with highly variable patterns of expression (Kisseberth et al. 1999). One luciferase transgenic line has been made utilizing a CMV-chicken β-actin promoter, but was noted to express the reporter only in a selected group of tissues that did not include brain (Cao et al. 2005). We chose the GAPDH promoter for control of the Renilla luciferase based on its frequent use in normalizing expression data, and recent studies indicate its utility for studies of chronic neurodegenerative disease in the mouse (Calvo et al. 2008). "
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