DCD – a novel plant specific domain in proteins involved in development and programmed cell death

Plant Molecular Biology, University of Frankfurt, Marie-Curie-Str, 9, 60439 Frankfurt, Germany.
BMC Bioinformatics (Impact Factor: 2.58). 02/2005; 6:169. DOI: 10.1186/1471-2105-6-169
Source: PubMed

ABSTRACT Recognition of microbial pathogens by plants triggers the hypersensitive reaction, a common form of programmed cell death in plants. These dying cells generate signals that activate the plant immune system and alarm the neighboring cells as well as the whole plant to activate defense responses to limit the spread of the pathogen. The molecular mechanisms behind the hypersensitive reaction are largely unknown except for the recognition process of pathogens. We delineate the NRP-gene in soybean, which is specifically induced during this programmed cell death and contains a novel protein domain, which is commonly found in different plant proteins.
The sequence analysis of the protein, encoded by the NRP-gene from soybean, led to the identification of a novel domain, which we named DCD, because it is found in plant proteins involved in development and cell death. The domain is shared by several proteins in the Arabidopsis and the rice genomes, which otherwise show a different protein architecture. Biological studies indicate a role of these proteins in phytohormone response, embryo development and programmed cell by pathogens or ozone.
It is tempting to speculate, that the DCD domain mediates signaling in plant development and programmed cell death and could thus be used to identify interacting proteins to gain further molecular insights into these processes.

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Available from: Tobias Doerks, Sep 27, 2015
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    • "contrasting chromosome 7H sibling classes within each F 3 family . Table 2 Genes in the HvNax3 intervals of rice and Brachypodium Rice or Brachypodium ortholog a ( barley marker ) Homology / inferred function LOC_Os06g07770 ( HYI ) Contains DCD domain . DCD proteins are a plant - specific group of proteins and presently have no ascribed function ( Tenhaken et al . 2005 ) LOC_Os06g07780 R - SNARE . Facilitate membrane fusion . Most similar to the three R - VAMP71 subfamily members of Arabidopsis ( http : / / www . tc . umn . edu / ~sande099 / atsnare . htm ) , which mediate vesicle fusion with the tonoplast ( Uemura et al . 2004 ; Carter et al . 2004 ) . Vesicle trafficking LOC_Os06g07800 Hypothetical "
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    ABSTRACT: Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na(+)) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection of the cultivated barley (H. vulgare ssp. vulgare) cultivar Barque (Barque-73, a moderate Na(+) excluder) attributed the control of the Na(+) exclusion trait from CPI-71284-48 to a single locus on the short arm of chromosome 7H, which was named HvNax3. The locus reduced shoot Na(+) accumulation by 10-25% in plants grown in 150 mM NaCl. Markers generated using colinearity with rice and Brachypodium, together with the analysis of introgression lines and F(2) and F(3) families, enabled HvNax3 to be mapped to a 1.3-cM interval. Genes from the corresponding rice and Brachypodium intervals encode 16 different classes of proteins and include several plausible candidates for HvNax3. The potential of HvNax3 to provide a useful trait contributing to salinity tolerance in cultivated barley is discussed.
    Functional & Integrative Genomics 05/2010; 10(2):277-91. DOI:10.1007/s10142-009-0153-8 · 2.48 Impact Factor
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    • "Additionally, many groups have reported expression of NAC genes in senescing leaves [80-84] and a NAC transcription factor (NAM-B1) isolated from wheat has been shown to regulate leaf senescence [85]. As for the N-rich genes, they encode a DCD (development and cell death) domain which is thought to be involved in the hypersensitive response and programmed cell death [62,78]. Based on the putative roles of the integrative genes, the possibility that the integrated pathway might transduce a PCD signal generated by prolonged ER stress and osmotic stress warrants further investigation. "
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    ABSTRACT: Despite the potential of the endoplasmic reticulum (ER) stress response to accommodate adaptive pathways, its integration with other environmental-induced responses is poorly understood in plants. We have previously demonstrated that the ER-stress sensor binding protein (BiP) from soybean exhibits an unusual response to drought. The members of the soybean BiP gene family are differentially regulated by osmotic stress and soybean BiP confers tolerance to drought. While these results may reflect crosstalk between the osmotic and ER-stress signaling pathways, the lack of mutants, transcriptional response profiles to stresses and genome sequence information of this relevant crop has limited our attempts to identify integrated networks between osmotic and ER stress-induced adaptive responses. As a fundamental step towards this goal, we performed global expression profiling on soybean leaves exposed to polyethylene glycol treatment (osmotic stress) or to ER stress inducers. The up-regulated stress-specific changes unmasked the major branches of the ER-stress response, which include enhancing protein folding and degradation in the ER, as well as specific osmotically regulated changes linked to cellular responses induced by dehydration. However, a small proportion (5.5%) of total up-regulated genes represented a shared response that seemed to integrate the two signaling pathways. These co-regulated genes were considered downstream targets based on similar induction kinetics and a synergistic response to the combination of osmotic- and ER-stress-inducing treatments. Genes in this integrated pathway with the strongest synergistic induction encoded proteins with diverse roles, such as plant-specific development and cell death (DCD) domain-containing proteins, an ubiquitin-associated (UBA) protein homolog and NAC domain-containing proteins. This integrated pathway diverged further from characterized specific branches of ER-stress as downstream targets were inversely regulated by osmotic stress. The present ER-stress- and osmotic-stress-induced transcriptional studies demonstrate a clear predominance of stimulus-specific positive changes over shared responses on soybean leaves. This scenario indicates that polyethylene glycol (PEG)-induced cellular dehydration and ER stress elicited very different up-regulated responses within a 10-h stress treatment regime. In addition to identifying ER-stress and osmotic-stress-specific responses in soybean (Glycine max), our global expression-profiling analyses provided a list of candidate regulatory components, which may integrate the osmotic-stress and ER-stress signaling pathways in plants.
    BMC Genomics 02/2007; 8(1):431. DOI:10.1186/1471-2164-8-431 · 3.99 Impact Factor
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    ABSTRACT: Modern concepts of programmed cell death, particularly the apoptosis in animals and plants are analyzed in this paper. A comparative characteristic of apoptosis in animal and plant cells taking into consideration the physiologic features of cells is presented. Necrosis as a form of pathological and not genetically programmed cell death is characterized. The significance (necessity) of apoptosis during the formation of a plant’s hypersensitive response and the role of programmed cell death under conditions of joint interrelations in the “pathogen-host” system are discussed.
    Cytology and Genetics 08/2010; 44(4):252-261. DOI:10.3103/S0095452710040110 · 0.38 Impact Factor
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