Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria.
ABSTRACT Propionibacterium acnes and Staphylococcus epidermidis have been recognized as pus-forming bacteria triggering an inflammation in acne. The present study was conducted to evaluate antimicrobial activities of Thai medicinal plants against these etiologic agents of acne vulgaris. Crude extracts were tested for antimicrobial activities by disc diffusion and broth dilution methods. The results from the disc diffusion method showed that 13 medicinal plants could inhibit the growth of Propionibacterium acnes. Among those, Senna alata, Eupatorium odoratum, Garcinia mangostana, and Barleria lupulina had strong inhibitory effects. Based on a broth dilution method, the Garcinia mangostana extract had the greatest antimicrobial effect. The MIC values were the same (0.039 mg/ml) for both bacterial species and the MBC values were 0.039 and 0.156 mg/ml against Propionibacterium acnes and Staphylococcus epidermidis, respectively. In bioautography assay, the Garcinia mangostana extract produced strong inhibition zones against Propionibacterium acnes. Antimicrobial activity from fractions of column chromatography revealed one of the active compounds in Garcinia mangostana could be mangostin, a xanthone derivative. Taken together, our data indicated that Garcinia mangostana had a strong inhibitory effect on Propionibacterium acnes and Staphylococcus epidermidis. Therefore, this plant would be an interesting topic for further study and possibly for an alternative treatment for acne.
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ABSTRACT: The antimicrobial and toxicological properties of the Australian essential oil, lemon myrtle, (Backhousia citriodora) were investigated. Lemon myrtle oil was shown to possess significant antimicrobial activity against the organisms Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, methicillin-resistant S. aureus (MRSA), Aspergillus niger, Klebsiella pneumoniae and Propionibacterium acnes comparable to its major component-citral. An in vitro toxicological study based on the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) cytotoxicity assay was performed. In vitro cytotoxicity testing indicated that both lemon myrtle oil and citral had a very toxic effect against human cell lines: HepG2 (a hepatocarcinoma-derived cell line); F1-73 (a fibroblast cell line derived from normal skin) and primary cell cultures of human skin fibroblasts. Cytotoxicity IC50 (50% inhibitory concentration) values ranged from 0.008 to 0.014% (w/v) at 4 h to 0.003-0.012% (w/v) at 24 h of exposure. The no-observed-adverse-effect level (NOAEL) for lemon myrtle oil was calculated as 0.5 mg/l at 24 h exposure and the RfD (reference dose) was determined as 0.01 mg/l. A product containing 1% lemon myrtle oil was found to be low in toxicity and could potentially be used in the formulation of topical antimicrobial products.Food and Chemical Toxicology 05/2002; 40(4):535-43. · 3.01 Impact Factor
Article: Naturally occurring antiacne agents.[show abstract] [hide abstract]
ABSTRACT: Antibacterial activity of various secondary metabolites from plants against Propionibacterium acnes was tested. In addition, the study of combinations of compounds to enhance the total activity against this follicular bacterium was investigated. A series of long-chain alcohols was studied in great detail to gain new insights into the role of the hydrophobic alkyl groups in the activity.Journal of Natural Products 02/1994; 57(1):9-17. · 3.29 Impact Factor
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ABSTRACT: From the Indian traditional medicines 78 plants were selected on the basis of their use in the treatment of infectious diseases. Different concentrations of 80% ethanol extracts were tested, using the agar dilution method, against four bacteria: Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa and, using the agar-well diffusion method, against two fungi: Candida albicans and Aspergillus niger. In the lowest tested concentration of 1.6 mg/ml, 10% of the plant extracts were active; 44% in a concentration of 6.25 mg/ml and 90% of the plant extracts were active against at least two bacteria in a concentration of 25 mg/ml. Only 13% of the plant extracts were active against at least one fungus in a concentration of 50 mg/ml.Journal of Ethnopharmacology 11/1997; 58(2):75-83. · 2.76 Impact Factor