Effect of quercetin supplementation on lung antioxidants after experimental influenza virus infection

Department of Respiratory Virology, VP Chest Institute, University of Delhi, Delhi 110-007, India.
Experimental Lung Research (Impact Factor: 1.41). 07/2005; 31(5):449-59. DOI: 10.1080/019021490927088
Source: PubMed


In the mice, instillation of influenza virus A/Udorn/317/72(H3N2) intranasally resulted in a significant decrease in the pulmonary concentrations of catalase, reduced glutathione, and superoxide dismutase. There was a decrease in vitamin E level also. These effects were observed on the 5th day after viral instillation. Oral supplementation with quercetin simultaneous with viral instillation produced significant increases in the pulmonary concentrations of catalase, reduced glutathione, and superoxide dismutase. However, quercetin did not reverse the fall in vitamin E level associated with the viral infection. It is concluded that during influenza virus infection, there is "oxidative stress." Because quercetin restored the concentrations of many antioxidants, it is proposed that it may be useful as a drug in protecting the lung from the deleterious effects of oxygen derived free radicals released during influenza virus infection.

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Available from: Pankaj Kumar, Sep 02, 2014
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    • "High mannose-binding lectins (HMBL) are powerful influenza and HIV inhibitors [154]. Rutin, quercetin, and related compounds, extracted from elderberry fruit (Sambucus nigra L.) [155] [156] [157] [158] [159] [160] [161] are other HA inhibitors. Xylopine and rosmaricin have an amine group that interacts with HA [162] [163]. "
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    Journal of preventive medicine and hygiene 12/2014; 55(4):109-129.
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    • "Docking studies have been performed using MOE 2008 . 10 . The crystal structure of HN protein ( PDB ID : 1USX ) was retrieved from Protein Data Bank ( http : / / www . rcsb . org / pdb / home / home . do ) ( Kumar et al . , 2005"
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    ABSTRACT: A series of novel substituted dihydropyrimidine and 5H-thiazolo [3, 2-a] pyrimidine derivatives were designed and synthesized as a potential target to discover drugs fighting against the viral diseases. The main objective of the present work is to carry out the QSAR studies for all the series of the compounds starting from 4a to 6j to find out their molecular descriptors and predict the biological properties. All of them are showing the best QSAR descriptors, hence chosen for the prediction of anti-viral activity against Newcastle disease virus (NDV). Initially their inhibitory activity was predicted by molecular docking of these compounds against haemaglutinin–neuraminidase (HN) protein using molecular operating environment (MOE) software. Based on the best affinity and highest docking scores 4b, 5b and 6b were assayed in vivo on NDV infected chicks and it was found that there is significant improvement in the survival of the chicks with the treatment (P < 0.05). 4b and 6b showed better curative effect than 5b at the dose concentration of 40 mg/kg body weight of chicks. The results from molecular docking study and biological assays can be inferred to consider these molecules as potential antiviral drugs.
    Antiviral Research 04/2012; Antiviral Research 95 (2012):118–127. · 3.94 Impact Factor
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    • "Bars=10 μm Naturwissenschaften (2011) 98:1019–1026 1023 Author's personal copy the SOD activity in the cell. Consistent with our results, the findings of Kumar et al. (2005) also reported decreased SOD activity levels in mice infected with influenza virus. Further, the decreased activities of CAT in virus infected tissues of birds over control chickens may indicate the accumulation of hydrogen peroxide. "
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    ABSTRACT: The aim of the present study was to investigate the effect of vitamin E on pro/anti-oxidant status in the liver, brain and heart of Newcastle disease virus (NDV) infected chickens. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione- S-transferase (GST) and the levels of reduced glutathione and malonaldehyde were estimated in selected tissues of uninfected, NDV-infected and NDV+vit. E-treated chickens. A significant increase in MDA levels in brain and liver ( p<0.05) was observed in NDV-infected chickens when compared to controls. The activities of SOD, CAT, GPx, GR, GST and levels of GSH were significantly ( p<0.05) decreased in brain and liver of NDV-infected chickens over controls. On the other hand, a significant decreased MDA levels and enhanced antioxidant enzyme activity levels were observed in NDV+vit. E-treated animals compared to NDV-infected chickens. Histopathological studies revealed that liver of NDV infected chicken shows focal coagulation and infiltration of hepatocytes, whereas neuronal necrosis and degeneration of Purkinje cells were observed in brain andmoderate infiltration of inflammatory cells was observed in heart. However such histological alterations were not observed in NDV+vit. E-treated animals. The results of the present study, thus demonstrated that antioxidant defense mechanism is impaired after the induction of NDV, suggesting its critical role in cellular injury in brain and liver. Further, the results also suggest that vitamin E treatment will ameliorate the antioxidant status in the infected animals. The findings could be beneficial to understand the role of oxidative stress in the pathogenesis of NDV and therapeutic interventions of antioxidants
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