Article

Organochlorine pesticide residues in European sardine, horse mackerel and Atlantic mackerel from Portugal.

Figueira da Foz Auction Center, Directorate General of Veterinary Services, Ministry of Agriculture, Rural Development and Fisheries, P-3080 Figueira da Foz, Portugal.
Food Additives and Contaminants (Impact Factor: 2.13). 08/2005; 22(7):642-6. DOI: 10.1080/02652030500136969
Source: PubMed

ABSTRACT This paper reports the results for the surveillance of nine organochlorine pesticides (HCH isomers (alpha, beta, e, gamma), p,p'-DDD, p,p'-DDT, p,p'-DDE, p,p'-DDD, HCB and aldrin) in muscle of three fish species, European pilchard (Sardina pilchardus), Atlantic horse mackerel (Trachurus trachurus) and Atlantic mackerel (Scomber scombrus). Analytical methodology included n-hexane extraction, clean-up with 2% deactivated Florisil, and quantification with gas chromatography-electron capture detection (GC-ECD). The highest mean concentrations were found for p,p'-DDT in sardine and mackerel at levels of 30.1 and 109.9 microg kg(-1), respectively, and for p,p'-DDD in horse mackerel at 51.9 microg kg(-1). Three species had higher levels for S-DDT than S-HCH. The estimated daily intake of organochlorine pesticides in the three species showed that in sardine, the highest EDIs were found for aldrin, at 1.8 ng kg(-1) bw day(-1), which represents 1.8% of the acceptable daily intake (ADI), and for ss-HCH, at 4.0 ng kg(-1) bw day(-1), representing 0.4% of ADI. Lowest values were found for Atlantic mackerel. Statistical analysis to determine the differences in mean concentrations of pesticides between species, and any correlation between groups of residues related with each one of the species, was undertaken.

0 Bookmarks
 · 
55 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Composite samples of Australian farmed Yellowtail Kingfish (Seriola lalandi) (YTKF) (n=27), Mulloway (Argyrosomus hololepidotus) (n=6) and manufactured feed (n=5) were analysed to benchmark levels of a broad range of residues and contaminants of potential public health and trade significance. A subset of these samples [YTKF (n=5), Mulloway (n=2) and feed (n=5)] was analysed for dioxins and polychlorinated biphenyls (PCBs). The mean concentration of dioxins in YTKF was 0.6 pg TEQ g(-1) (range 0.22-0.8) and in Mulloway was 0.16 pg TEQ g(-1) (range 0.16-0.16). The mean concentration of dioxins and dioxin-like PCBs in YTKF was 2.6 pg TEQ g(-1) (range 1.4-3.5), while Mulloway had a mean concentration of 0.67 pg TEQ g(-1) (range 0.57-0.76). The mean concentration of PCBs in YTKF was 21 μg kg(-1) (range 8.6-29) and in Mulloway was 5.4 μg kg(-1) (mean 4.7-6). The mean concentration of dioxin-like PCBs in YTKF was 2.1 pg TEQ g(-1) (range 1.2-2.8) and in Mulloway was 0.51 pg TEQ g(-1) (range 0.41-0.61). The mean mercury concentration in YTKF was 0.03 mg kg(-1) (range 0.02-0.05) and in Mulloway it was 0.02 mg kg(-1) (range 0.02-0.04). There were no detectable levels of any pesticide or antimicrobial compounds in any sample of YTKF or Mulloway. Attention is drawn to technical differences in port of entry testing programs such as sampling strategies, portion tested, laboratory methodology, residue definitions and reporting conventions that exporters' products may be subject to. All residues and contaminants were either undetectable or present at very low levels when judged against Australian, Japanese and European Union regulatory standards (where set).
    Chemosphere 12/2011; 86(7):709-17. · 3.14 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Fifteen seaweeds belong to 13 families and 6 orders of the rhodophyta were sampled for one year from April 2007 to March 2008 along the southwest coast of India (Indian Ocean). The species were examined forin vitro antimicrobial activity against six pathogenicVibrio strains isolated from moribund tiger shrimp (Penaeus monodon), six type cultures (Microbial Type Culture Collection, MTCC) of prominent shrimpVibrio pathogens, 10 multidrug resistant clinical pathogens, four species ofCandida obtained from pulmonary TB patients and four species of plant pathogenic fungi to evaluate their potency to be used as natural antibiotics in pharmaceutical and agriculture field. Bioactivity was analyzed from crude extract of fresh and dried samples prepared from different polar and nonpolar solvents. Of these, four species of red algae (Asparagopsis taxiformis, Laurencia ceylanica, Laurencia brandenii, Hypnea valentiae) were found to be highly active. Broadest and highest activity was observed in the crude extract ofA. Taxiformis. Among the pathogens tested, shrimp pathogenicVibrios were the most susceptible organisms while phytopathogens were found to be little resistant. In the present study, methanol was found to be the best solvent for extracting antimicrobial metabolites from dried samples rather than fresh. Seasonal variation in the antimicrobial activity was observed with higher level of activity recorded fromA. Taxiformis between December and January. The active principle ofA. Taxiformis was purified in column chromatography, TLC and reverse phase HPLC. The individual HPLC peaks were subsequently tested against a panel of pathogenic microorganisms and the active constituent was identified by GC-MS. The antimicrobial profile ofA. Taxiformis suggested that lipophilic compound which was primarily composed of pyrrole-2-carboxylic acid, pentadecanoic acid and octadecanoic acid might have functional role in the chemical defence against microbial invasion and these compounds could be utilized for the development of medically potential products. Key wordsseaweed extract- Asparagopsis taxiformis -antimicrobial activity-shrimpVibrios -mycotoxic activity-phytopathogens
    Annals of Microbiology 01/2009; 59(2):207-219. · 1.55 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Pesticides are widely used in agriculture and can be transferred to animals in a number of ways. Consequently, reliable analytical methods are required to determine pesticide residues in foods of animal origin. The present review covers published methods and research articles (1990-2010) in which pesticide residues have been extracted from meat and meat products, milk and dairy products, fish and seafood, and eggs, then cleaned up, and isolated by chromatographic techniques to be identified and quantified by various detection methods. Recovery rates, quantification limits, the matrix effect and related parameters have all been considered. Lastly, future developments in this field are outlined.
    Journal of Chromatography A 02/2011; 1218(8):1021-36. · 4.61 Impact Factor